| 孙秀贤,孟昭福,曹雪雯,王腾,胡啸龙,王新欣,刘欣.漆酶增强黄麻去除水中亚甲基蓝的研究[J].农业环境科学学报,2021,40(7):1548-1556. |
| 漆酶增强黄麻去除水中亚甲基蓝的研究 |
| Laccase mediated jute enhancement for the removal of methylene blue from water |
| 投稿时间：2020-12-13 |
| DOI：10.11654/jaes.2020-1434 |
| 中文关键词: 漆酶-黄麻复合材料 吸附 酶解 亚甲基蓝 再生 |
| 英文关键词: laccase-jute composite adsorption enzymolysis methylene blue regeneration |
| 基金项目:国家自然科学基金项目（41271244）；陕西省重点研发计划项目（2017SF-385） |
|
| 摘要点击次数: 2000 |
| 全文下载次数: 2208 |
| 中文摘要: |
| 以段状黄麻（SJ）为基质，通过酶固定化方法，制备了系列漆酶-黄麻复合材料（0.1% L-SJ、0.5% L-SJ、1% L-SJ、5% L-SJ），通过批处理法研究了漆酶给酶量、温度、pH、离子强度及其再生对亚甲基蓝（MB）去除性能的影响，并通过红外光谱、扫描电镜及表面电荷特征等表征其官能团、表面形貌和电荷变化以探讨其对MB的去除机制。结果表明：漆酶的负载增大了黄麻对MB的去除，随漆酶给酶量的增加，MB去除率顺序为1% L-SJ > 5% L-SJ > 0.5% L-SJ > 0.1% L-SJ > SJ，呈现先增加后减小的规律；5种材料对MB的去除均符合Sips吸附等温模型，1% L-SJ具有最高的去除率达95.12%，拟合吸附量（qS）为1 097.11 mmol·kg-1；5种材料对MB的去除均为自发过程，温度的升高、离子强度的增大和pH的降低均不利于MB去除；1% L-SJ经再生4次后仍保持76.66%的去除率。漆酶-黄麻复合材料对MB的去除机制是黄麻吸附与漆酶酶解的共同作用，黄麻吸附是主要形式，贡献率为91.12%，而漆酶的辅助作用贡献率为8.88%。黄麻吸附机制主要为静电相互作用和氢键作用，漆酶主要通过酶解和改变黄麻结构的形式增强对MB的去除。 |
| 英文摘要: |
| In this study, laccase was loaded in a segment jute（SJ）matrix, and a series of laccase-jute composite materials（0.1%L-SJ, 0.5%L-SJ, 1%L-SJ, 5%L-SJ）were produced. The effects of laccase dosage, temperature, pH, ionic strength, and regeneration on the efficiency to remove methylene blue（MB）were studied using the batch method. Furthermore, Fourier Transform Infrared Spectroscopy, Scanning Electronic Microscopy, and the surface charge were assessed to characterize and explore the mechanism of MB removal. The results indicated that the laccase-loaded jute exhibited enhanced MB removal ability compared with that of raw jute. The order of MB removal rate was 1%L-SJ > 5%L-SJ > 0.5%L-SJ > 0.1%L-SJ>SJ, exhibiting a trend of first increasing and then decreasing. Additionally, the MB removal by the five materials conformed to the Sips adsorption isotherm model, where 1%L-SJ exhibited the highest removal rate of 95.12% and the fitted adsorption capacity（qS）was 1 097.11 mmol·kg-1. Furthermore, the removal process was spontaneous, and the increase in temperature and ionic strength and a decrease in pH were not conducive to MB removal. Finally, 1%L-SJ maintained a removal rate of 76.66% after being regenerated four times. The mechanism of MB removal by the laccase-jute composite was found to the synergistic action of jute adsorption and laccase enzymatic hydrolysis. Jute adsorption contributed to 91.12% of MB removal, while enzymatic hydrolysis of laccase contributed to 8.88% of MB remval. The adsorption mechanisms of jute mainly involved electrostatic interaction and hydrogen bonding, whereas laccase improved the MB removal by enzymatic hydrolysis and altering the jute structure. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
