| 侯明,霍岩,张志专,韦明奉.土壤外源钒施加对玉米中钒积累、亚细胞分布和非蛋白巯基含量的影响[J].农业环境科学学报,2020,39(5):964-972. |
| 土壤外源钒施加对玉米中钒积累、亚细胞分布和非蛋白巯基含量的影响 |
| Effects of exogenous vanadium stress on vanadium accumulation and subcellular distribution, and non-protein thiol content in maize(Zea mays L.)crops |
| 投稿时间：2020-01-15 |
| DOI：10.11654/jaes.2020-0065 |
| 中文关键词: 钒 玉米幼苗 积累 亚细胞分布 非蛋白巯基化合物 |
| 英文关键词: vanadium maize (Zea mays L.)seedling accumulation subcellular distribution non-protein thiol compound |
| 基金项目:国家自然科学基金项目（41561077） |
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| 摘要点击次数: 2449 |
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| 中文摘要: |
| 本研究旨在探讨玉米对钒（V）的耐受性机制及富集机理。采用差速离心法和DTNB[5，5'-二硫代双（2-硝基苯甲酸）]比色法，研究了不同V浓度（0、100、200、300、500 mg·kg-1）胁迫下，V在玉米中的积累、亚细胞分布和非蛋白巯基物质对V胁迫的响应。结果表明，V在玉米各部位中积累顺序为：根 > 叶 > 茎 >> 子实，其中74.8%~95.6%的V富集于根部。随着V胁迫浓度的增加，V的富集系数（BF）从0.061增加到0.306，而转运系数（TF）从0.336下降到0.108，从而降低了过量V对玉米茎叶的毒性。V主要积累于植物亚细胞组分的细胞壁（F1）和可溶性组分（F4）中，两者在茎叶中占总累积量的60.76%~75.75%，在根中占总累积量的82.66%~87.02%。土壤中一定的V浓度（V≤300 mg·kg-1）可促进玉米幼苗体内非蛋白巯基（NPT）和植物螯合肽（PCs）的合成，植物也通过消耗谷胱甘肽（GSH）来合成植物螯合肽（PCs），从而降低V的毒性。因此，玉米幼苗抗V毒性的机制包括细胞壁沉积和重金属分区，以及非蛋白巯基化合物的合成。 |
| 英文摘要: |
| The purpose of the present study is to investigate vanadium (V)-tolerance and-enrichment mechanisms in maize (Zea mays L.) crops. Maize seedlings were treated with varying concentrations of V (0, 100, 200, 300, and 500 mg·kg-1)to investigate the differences in bioaccumulation and subcellular distribution of V and the responses of non-protein thiols (NPTs)to V exposure in maize seedlings by differential centrifugation and 5,5'-dithio bis-(2-nitrobenzoic acid)colorimetry, respectively. The findings revealed that V bioaccumulation in different parts of maize plants decreased in the following order:roots > leaves > stems > grains; 74.8%~95.6% of V was accumulated in the roots. With an increase in V concentration, the bioconcentration factor increased from 0.061 to 0.306, whereas the translocation factor decreased from 0.336 to 0.108, thereby reducing the toxicity of excessive V to the stems and leaves of maize seedlings. V was mainly accumulated in the cell wall (F1)and soluble component (F4)of plant, both of which account for 60.76% to 75.75% of the total accumulation in the shoots, and from 82.66% to 87.02% of the total accumulation in the roots. V at certain concentrations in soil (≤ 300 mg·kg-1)could promote the synthesis of NPTs and phytochelatins (PCs); plants utilize glutathione to synthesize PCs, thereby reduce the toxicity of V. Thus, the strategies employed by maize seedlings against V toxicity involve cell wall deposition, heavy metal compartmentalization, and thiol compound synthesis. |
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