底泥中三氯生残留对轮叶黑藻的生态毒性效应

张立娜; 王芷茵; 安婧

文章摘要

张立娜,王芷茵,安婧.底泥中三氯生残留对轮叶黑藻的生态毒性效应[J].农业环境科学学报,2019,38(9):2057-2065.

底泥中三氯生残留对轮叶黑藻的生态毒性效应

Ecotoxicological effects of triclosan on Hydrilla verticillata in sediments

投稿时间：2019-01-13

DOI：10.11654/jaes.2019-0050

中文关键词: 三氯生轮叶黑藻底泥暴露生态毒性

英文关键词: triclosan Hydrilla verticillata sediment exposure ecological toxicity

基金项目:国家自然科学基金项目（31971515）

作者	单位	E-mail
张立娜	中国科学院沈阳应用生态研究所污染生态与环境工程重点实验室, 沈阳 110016 中国科学院大学, 北京 100049
王芷茵	东北大学资源与土木工程学院, 沈阳 110016
安婧	中国科学院沈阳应用生态研究所污染生态与环境工程重点实验室, 沈阳 110016	anjing@iae.ac.cn

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中文摘要:

为探讨三氯生（triclosan，TCS）低浓度长期暴露对水生生态系统的毒性效应，本文以典型沉水植物——轮叶黑藻为研究对象，利用HPLC-MS、UV-VIS等分析技术，研究了0.05~0.5 mg·kg^-1 TCS底泥暴露28 d后轮叶黑藻体内TCS的残留浓度及叶绿素、可溶性蛋白、抗氧化酶活性的变化。结果表明，在处理组中轮叶黑藻叶片中TCS的含量在暴露初期（14 d）呈下降趋势，随着暴露时间延长，叶片中TCS含量逐渐升高，暴露28 d时，0.5 mg·kg^-1 TCS处理组中轮叶黑藻叶片中TCS浓度高达2.16 mg·kg^-1。TCS暴露周期内，轮叶黑藻叶片中叶绿素含量呈持续下降趋势，可溶性蛋白含量呈先促进后抑制的趋势，而其茎部可溶性蛋白含量则始终呈抑制状态。此外，TCS胁迫可显著影响轮叶黑藻叶片和茎部的过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和过氧化物酶（POD）活性，对轮叶黑藻的抗氧化系统造成不可逆的损伤。研究结果为评估水体环境中TCS的生态风险提供了基础数据和理论依据。

英文摘要:

Chronic toxicity of triclosan (TCS)on submerged plant Hydrilla verticillata in sediments with 0.05~0.5 mg·kg^-1 TCS was studied. TCS concentration, chlorophyll content, soluble protein content, and antioxidant enzyme activities in H. verticillata were determined using HPLC-MS and UV-VIS techniques. The results showed that TCS concentration in the leaves of H. verticillata in treatment groups was low in the early exposure stage (14 d). However, bioaccumulation of TCS increased with exposure time; its concentration in the leaves was 2.16 mg·kg^-1 for 0.5 mg·kg^-1 TCS sediment in 28 d. During the TCS exposure period, the chlorophyll content in the leaves of H. verticillata decreased continuously in the TCS treatment groups in comparison to the control group. The soluble protein content in H. verticillata leaves first increased and then declined with increasing exposure time, whereas in the plant stems it was inhibited significantly during the entire TCS exposure period. Furthermore, TCS had significant influences on H. verticillata antioxidant enzyme system (CAT, SOD, and POD activities), which led to plant damage. The current findings will provide fundamental data for evaluating the risk and toxicity of TCS to the aquatic ecosystems.

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