| 车涛,张丽萍,金竹萍,裴雁曦.硫化氢对镉胁迫下大白菜幼苗光合生理的影响[J].农业环境科学学报,2019,38(5):1008-1016. |
| 硫化氢对镉胁迫下大白菜幼苗光合生理的影响 |
| Hydrogen sulfide regulates the photosynthetic physiology of Brassica pekinensis (Lour.) Rupr. under cadmium stress |
| 投稿时间：2018-11-10 |
| DOI：10.11654/jaes.2018-1417 |
| 中文关键词: 大白菜 硫化氢 光合作用 镉 |
| 英文关键词: Chinese cabbage hydrogen sulfide photosynthesis cadmium |
| 基金项目:国家自然科学基金面上项目（31672140） |
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| 摘要点击次数: 2448 |
| 全文下载次数: 2730 |
| 中文摘要: |
| 为了研究H2S对镉（Cd）胁迫下大白菜[Brassica pekinensis（Lour.）Rupr.]幼苗光合生理的调节作用，通过生理生化实验检测Cd胁迫和H2S对幼苗光合特性指标和Rubp羧化酶（Ribulose-1，5-hisphpsphate carboxylase）活性的影响，利用荧光定量-PCR检测Rubp小亚基编码基因RBCS表达水平的变化。结果表明：5 mmol·L-1 Cd胁迫会显著抑制大白菜幼苗的生长，降低叶片叶绿素含量、叶绿素荧光参数（Fv/F0、Fv/Fm、PIabs）和净光合速率（Pn）；5 μmol·L-1 NaHS预处理可以明显缓解Cd胁迫对上述指标的抑制作用，增加Cd胁迫下RBCS的表达量，促进非Cd胁迫下Rubp羧化酶的活性；0.1 mmol·L-1次牛磺酸（H2S清除剂）与Cd共同处理对幼苗的损伤比单独Cd胁迫更强。以上结果表明，H2S预处理可以提高大白菜幼苗的光合作用强度，有效缓解Cd胁迫对其造成的损伤，维持Cd胁迫下大白菜幼苗的生长。 |
| 英文摘要: |
| To study the function of hydrogen sulfide (H2S) on the photosynthetic physiology of Chinese cabbage[Brassica pekinensis (Lour.) Rupr.] seedlings under cadmium (Cd) stress, we examined the effects of Cd stress and H2S on photosynthetic characteristics, ribulose-1,5-hisphpsphate (Rubp) carboxylase activity, and the expression level of RBCSes (small subunits of Rubp) using physiological, biochemical, and real-time quantitative PCR analyses. Seedling growth, chlorophyll content, photosynthetic fluorescence parameters (e.g., Fv/F0, Fv/Fm, and PIabs), and net photosynthetic rate (Pn) were inhibited considerably under 5 mmol·L-1 Cd stress, whereas 5 μmol·L-1 NaHS pretreatment alleviated the effects of Cd stress physiologically and up-regulated the mRNA levels of RBCSes under Cd stress. H2S promoted the activity of seedling Rubp carboxylase without Cd stress. In contrast, the presence of 0.1 mmol·L-1 hypotaurine (H2S scavenger) aggravated the inhibition effects of Cd stress on the seedlings. Thus, H2S enhanced the photosynthesis of seedlings to alleviate the damage caused by Cd stress and to maintain the growth of Chinese cabbage under Cd stress. |
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