| 韩娜,陶宗娅,代文秀,黄攀,刘巧,赖金龙,吴国,卢红.淡紫拟青霉A10的促生特性及其发酵液对印度芥菜幼苗蓄积Cs+的影响[J].农业环境科学学报,2019,38(3):485-493. |
| 淡紫拟青霉A10的促生特性及其发酵液对印度芥菜幼苗蓄积Cs+的影响 |
| Plant growth-promoting characteristics of A10(Paecilomyces lilacinus)and effects of its fermentation broth on Cs+ accumulation in Brassica juncea L. seedlings |
| 投稿时间：2018-04-07 |
| DOI：10.11654/jaes.2018-0450 |
| 中文关键词: 淡紫拟青霉A10 铯 印度芥菜幼苗 植物-微生物互作 |
| 英文关键词: Paecilomyces lilacinus cesium indian mustard (Brassica juncea L.)seedling plant-microbial interaction |
| 基金项目:四川师范大学重点培育项目（16ZP09）；四川师范大学实验技术课题（SYJS2016-08）；地方高校国家级大学生创新创业训练计划项目（201710636014） |
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| 中文摘要: |
| 从种植印度芥菜的盆栽土壤（ρ[Cs+]为10 mmol·kg-1）中筛选出淡紫拟青霉（A10），通过测试分析A10溶磷、解钾、分泌植物生长素（IAA）类似物及其发酵液对印度芥菜幼苗蓄积Cs+的影响，研究A10的植物促生特性及其发酵液在印度芥菜蓄积Cs+过程中的作用，为植物-微生物互作在Cs+污染环境修复中的应用及其互作机制研究提供实验依据。结果显示：A10对Cs+的耐受性和富集能力强，ρ（Cs+）为50~100 mg·L-1时A10的溶磷能力被显著抑制（P<0.05）；Cs+处理及外源色氨酸均可显著诱导A10分泌IAA类似物；用1/2 Hoagland营养液稀释A10发酵液后再配制ρ（Cs+）为25~100 mg·L-1的处理液，用其培养印度芥菜，ρ（Cs+）为0 mg·L-1（CK）时A10发酵液对印度芥菜幼苗根的生长呈现"低促高抑"的生长素效应，稀释35倍时的促根效果最佳；A10发酵液稀释5、35倍和55倍条件下，ρ（Cs+）为100 mg·L-1时幼苗地上部Cs+蓄积量均显著升高，其中稀释35倍时Cs+蓄积量达最大值，地上部为8.86 mg·g-1DW，地下部为16.76 mg·g-1 DW，印度芥菜幼苗蓄积Cs+与其吸收K+呈显著的负相关性。研究表明，淡紫拟青霉A10具有显著的植物促生特性，A10发酵液有利于提高印度芥菜幼苗对外源Cs+的蓄积。 |
| 英文摘要: |
| In the present study, a strain of the fungus Paecilomyces lilacinus, named A10, was isolated from potted soil with a cesium (Cs+) content (ρ[Cs+]) of 10 mmol·kg-1. A10 was studied in order to investigate its plant growth-promoting characteristics and the effects of A10 fermentation broth on Cs+ bioaccumulation in Indian mustard (Brassica juncea L.) seedlings, by measuring the secretion of auxin (IAA) analogs, the content of soluble phosphorus, the effective potassium in media with different Cs+ concentrations, and the Cs+ content in seedlings. The following results were obtained. A10 had strong tolerance and Cs+-enrichment traits, although the phosphorus solubilization ability of A10 was significantly inhibited when the ρ[Cs+] in the medium was 50~100 mg·L-1 (P<0.05). Treatments in which either Cs+ or exogenous tryptophan was added to the medium could significantly increase the secretion of IAA analogs induced by A10. A10 fermentation broth was diluted (dilution ratio 5x, 35x, 55x) with 1/2-strength Hoagland nutrient solution, formulated to yield solutions containing ρ (Cs+) 25~100 mg·L-1, and then used as culture solutions to cultivate Brassica juncea seedlings. At ρ (Cs+) 0 mg·L-1 (CK), the dilute solution of A10 fermentation broth clearly affected root growth, and the secretion of IAA initially increased and subsequently decreased. Optimal root-promoting effects were observed in seedlings treated with the 35x dilutions of fermentation broth. When seedling were cultured in 5x, 35x, and 55x diluted broth containing ρ (Cs+) 100 mg·L-1, the amounts of Cs+ bioaccumulated increased significantly in the aboveground parts of seedlings, with maximum values of 8.86 mg·g-1 DW in the aboveground parts and 16.76 mg·g-1 DW in the belowground parts being recorded in seedlings cultured in the 35x diluted broth. Furthermore, we detected a significant negative correlation between the accumulated Cs+ and K+ content in the aboveground parts of seedlings. Collectively, these results indicate that A10 has distinct plant growth-promoting properties, whereas treatments with A10 fermentation broth can promote Cs+ bioaccumulation in Indian mustard seedlings. |
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