文章摘要
马珊珊,赵光辉,常文越,刘智,张依然,陈红星,谢凌天.Se(Ⅳ)和Se(Ⅵ)在斑马鱼组织中的累积及对抗氧化系统的影响[J].农业环境科学学报,2019,38(1):51-59.
Se(Ⅳ)和Se(Ⅵ)在斑马鱼组织中的累积及对抗氧化系统的影响
Selenium accumulation and its effects on the antioxidant system of zebrafish(Danio rerio)exposed to selenite and selenate
投稿时间:2018-03-05  
DOI:10.11654/jaes.2018-0287
中文关键词: 斑马鱼  抗氧化系统  过氧化氢酶  总抗氧化能力  Na+/K+-ATPase
英文关键词: zebrafish  antioxidant system  catalase(CAT)  total antioxidant capacity(T-AOC)  Na+/K+-ATPase
基金项目:沈阳市科学事业费科技项目(150057);国家自然科学基金项目(31270549);中国科学院百人计划项目(Y2SBR111SS);现代农业产业技术体系专项资金(CARS-46)
作者单位E-mail
马珊珊 沈阳环境科学研究院, 沈阳 110167
辽宁省城市生态重点实验室, 沈阳 110167
沈阳工程学院辽宁省洁净燃烧发电与供热技术重点实验室, 沈阳 110136
中国科学院沈阳应用生态研究所, 沈阳 110016 
 
赵光辉 沈阳环境科学研究院, 沈阳 110167
辽宁省城市生态重点实验室, 沈阳 110167 
 
常文越 沈阳环境科学研究院, 沈阳 110167
辽宁省城市生态重点实验室, 沈阳 110167 
changwenyue@163.com 
刘智 沈阳环境科学研究院, 沈阳 110167
辽宁省城市生态重点实验室, 沈阳 110167 
 
张依然 沈阳环境科学研究院, 沈阳 110167
辽宁省城市生态重点实验室, 沈阳 110167 
 
陈红星 华南师范大学环境研究院环境理论化学教育部重点实验室, 广州 510006  
谢凌天 华南师范大学环境研究院环境理论化学教育部重点实验室, 广州 510006  
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中文摘要:
      以斑马鱼为受试材料,研究了Se(Ⅳ)和Se(Ⅵ)在斑马鱼组织中的累积及对抗氧化系统的影响。试验分3组,分别为对照组、Se(Ⅳ)处理组(500 μg· L-1)和Se(Ⅵ)处理组(5000 μg·L-1),处理28 d后,测定斑马鱼脑、鳃、肝、肠和肌肉组织中硒的累积以及过氧化氢酶(CAT)活性、总抗氧化能力(T-AOC)和Na+/K+-ATPase活性。结果显示,与对照组相比,硒处理组斑马鱼各组织中呈现不同程度的硒累积,肝组织中硒累积量最高,肠组织中CAT活性、鳃和肠组织中T-AOC活性以及鳃和肝组织中Na+/K+-ATPase活性均显著降低。Se(Ⅳ)与Se(Ⅵ)处理组相比,斑马鱼肝和鳃组织中硒累积量显著升高,肝组织中CAT活性显著下降13%,T-AOC活性显著升高1.8倍。研究结果表明Se(Ⅳ)比Se(Ⅵ)生物可利用性更高,硒累积导致斑马鱼组织发生氧化胁迫,试验为进一步从抗氧化系统的角度研究硒暴露对鱼类等水生生物的毒性作用机制奠定了基础。
英文摘要:
      This study aimed to explore the effects of Se(Ⅳ) and Se(Ⅵ) exposure on the antioxidant system and Se accumulation in zebrafish(Danio rerio) tissues. The study organisms were divided into three groups, i.e., control group, Se(Ⅳ) -exposed group(500 μg·L-1), and Se(Ⅵ) -exposed group(5000 μg·L-1). The Se accumulation, catalase(CAT), total antioxidant capacity(T-AOC), and Na+/K+-ATPase activity were determined in the brain, gill, liver, intestine, and muscle tissues of zebrafish after exposure to both Se species for 28 days. The results showed different extents of Se accumulation in fish tissues from the Se-exposed groups compared with the control treatment. The accumulation of Se in the liver was the highest. CAT activity in the intestine, T-AOC activity in the gills and intestine, and Na+/K+-ATPase activity in the gills and liver tissues were significantly decreased. As compared with the Se(Ⅵ) treatment, Se accumulation in the liver and gill tissues of fish was significantly elevated in the Se(Ⅳ) treatment. CAT activity in the liver was significantly decreased 13%. However, the T-AOC was significantly increased 1.8 times. These results demonstrated that Se(Ⅳ) is more bioavailable than Se(Ⅵ). Se accumulation caused oxidative stress in zebrafish tissues. Our findings represent a progress in research on the toxicological mechanism of Se exposure in fish and other aquatic organisms from the aspects of the antioxidant defense system.
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