| 杨婧,郭楚玲,刘沙沙,党志,卢桂宁.邻苯二甲酸酯降解菌的筛选、降解特性及土壤修复研究[J].农业环境科学学报,2018,37(5):933-940. |
| 邻苯二甲酸酯降解菌的筛选、降解特性及土壤修复研究 |
| Isolation and characterization of phthalate-degrading bacterial strain, and its application in phthalate-contaminated soil |
| 投稿时间：2017-12-07 |
| DOI：10.11654/jaes.2017-1689 |
| 中文关键词: DEHP 分枝杆菌 代谢途径 污染土壤 |
| 英文关键词: DEHP Mycobacterium sp. degradation pathway contaminated soil |
| 基金项目:广东省科技项目（2014A020217002，2016B020242004） |
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| 摘要点击次数: 2720 |
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| 中文摘要: |
| 为寻找高效邻苯二甲酸二（2-乙基己基）酯（DEHP）降解菌，采用富集培养法从城市污水处理厂活性污泥中分离筛选出一株DEHP降解菌并命名为ASW6D。通过扫描电镜、16S rRNA同源性序列分析，初步将菌株ASW6D鉴定为分枝杆菌属（Mycobacterium sp.）。菌株ASW6D可在较宽温度（20~40℃）和pH （5~10）范围下高效降解DEHP，其最适生长降解条件为30℃、pH 8.0，3 d内可将初始浓度为500 mg·L-1的DEHP降解82.87%。进一步采用GC-MS分析DEHP降解的中间产物，推测出DEHP的生物代谢途径为先通过β-氧化缩短DEHP侧链，生成邻苯二甲酸二丁酯（DBP），再将DBP转化为邻苯二甲酸（PA）。将菌株ASW6D接种到DEHP污染的土壤，可将土壤中DEHP去除率提高58.67%，表明ASW6D在PAEs污染环境生物修复方面的应用具有一定的潜力。 |
| 英文摘要: |
| Di(2-ethylhexyl) phthalate(DEHP), one of the most widely used phthalates in China, is easily released into the environment. In the present study, we isolated a high-efficient DEHP-degrading bacterial strain(named ASW6D) enriched from the activated sludge of municipal sewage treatment plant. Based on its morphology and 16S rRNA sequence analysis, the bacterial strain was identified as Mycobacterium sp.. This species can efficiently degrade DEHP at a wide range of temperature(20~40℃) and pH(5~10). The optimal temperature and pH for the growth of ASW6D were 30℃ and 8.0, respectively. Under optimal conditions, ASW6D degraded 500 mg·L-1 DEHP by more than 82.87% within 3 d. Further, ASW6D also utilized dimethyl phthalate(DMP) and dibutyl phthalate(DBP) as carbon sources. The metabolites of DEHP degradation were further analyzed by Gas Chromatography-Mass Spectrometer(GC-MS). The metabolic pathway of DEHP was as follows:initially the side chain of DEHP was shortened by β-oxidation, and then DBP was formed; DBP was then converted to phthalic acid(PA) by hydrolysis. The inoculation of ASW6D into the contaminated soil increased the removal rate of DEHP by 58.67%. This indicates that ASW6D has the potential for bioremediation of phthalate acid ester(PAE)-contaminated soil. |
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