| 顾平,周启星,王鑫,伍跃辉,陈威,张倩茹,龚起,周爱申.一株土著B[a]P降解菌的筛选及降解特性研究[J].农业环境科学学报,2018,37(5):926-932. |
| 一株土著B[a]P降解菌的筛选及降解特性研究 |
| The screening-out and degradation character of an indigenous benzo[a]pyrene-degrading bacteria |
| 投稿时间：2017-07-06 |
| DOI：10.11654/jaes.2017-0954 |
| 中文关键词: PAHs B[a]P 污染土壤 生物降解 |
| 英文关键词: polycyclic aromatic hydrocarbons (PAHs) benzo[a]pyrene (B[a]P) contaminated soil biodegradation |
| 基金项目:中央高校基本科研业务费专项资金项目 |
| 作者 | 单位 | E-mail | | 顾平 | 黑龙江省环境监测中心站, 哈尔滨 150056
中国科学院沈阳应用生态研究所, 沈阳 110016 | | | 周启星 | 南开大学环境科学与工程学院, 环境污染过程与基准教育部重点实验室/天津市城市生态环境修复与污染防治重点实验室, 天津 300071 | zhouqx@nankai.edu.cn | | 王鑫 | 南开大学环境科学与工程学院, 环境污染过程与基准教育部重点实验室/天津市城市生态环境修复与污染防治重点实验室, 天津 300071 | | | 伍跃辉 | 黑龙江省环境监测中心站, 哈尔滨 150056 | | | 陈威 | 黑龙江省环境监测中心站, 哈尔滨 150056 | | | 张倩茹 | 中国科学院沈阳应用生态研究所, 沈阳 110016 | | | 龚起 | 黑龙江省环境应急与事故调查中心, 哈尔滨 150090 | | | 周爱申 | 黑龙江省环境监测中心站, 哈尔滨 150056 | |
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| 中文摘要: |
| 通过在液体培养基中添加苯并[a]芘（B[a]P）作为唯一碳源反复驯化培养，从长期受石油烃和多环芳烃（PAHs）污染的土壤中分离出8株能够降解B[a]P的细菌，其中一株细菌（命名为BB-1）具有最大降解效果。采用16sRNA基因测序结果表明，BB-1与巨大芽孢杆菌（Bacillus megaterium）同源性为100%。为了研究BB-1的降解特性，在pH7.0、30℃条件下培养8 d，菌株B[a]P的降解率高达66.36%。为了考察培养基初始pH和外加蔗糖浓度对BB-1降解B[a]P的影响，30℃下振荡培养8 d，当设置培养基初始pH为4.0、6.0、8.0和10.0时，BB-1对B[a]P的降解率分别为12.14%、39.61%、55.21%和30.03%，可见pH为8.0时其降解效果最优；当添加0.1%蔗糖和0.5%蔗糖为外加碳源时，菌株BB-1对B[a]P的降解率分别为70.56%和74.89%，表明0.5%蔗糖的降解效果最优。 |
| 英文摘要: |
| Eight bacteria strains were isolated from the soil contaminated with petroleum hydrocarbons and polycyclic aromatic hydrocarbons(PAHs) using repeated subcultures in liquid medium with added benzo[a]pyrene(B[a]P) as the sole carbon source. One of the bacterial strains named BB-1 had an optimal degradation effect(100% homologous to Bacillus megaterium) through the 16sRNA gene sequencing method. In order to explore the degradation character of BB-1,the strain was cultured under pH=7.0, 30℃ for 8 days, after which the B[a]P degradation rate reached a maximum of 66.36%. In order to investigate the degradation rate of BB-1 under varying conditions of initial pH and additional sucrose concentration in the culture medium, the incubation was carried out at 30℃ for 8 days, with various values of initial pH including 4.0, 6.0, 8.0, and 10.0. The results showed that the degradation rates of B[a]P were 12.14%, 39.61%, 55.21%, and 30.03%, respectively. In other words, the highest degradation efficiency was achieved for pH 8.0. With the addition of 0.1% and 0.5% sucrose as an additional carbon source, the degradation rate of B[a]P reached 70.56% and 74.89%, respectively. It was thus clear that the highest degradation efficiency occurred in the media with the addition of 0.5% sucrose. |
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