几种丛枝菌根真菌对菲污染土壤中球囊霉素含量的影响

杨振亚; 阙弘; 朱雪竹; 周紫艳; 陈爽; 凌婉婷

文章摘要

杨振亚,阙弘,朱雪竹,周紫艳,陈爽,凌婉婷.几种丛枝菌根真菌对菲污染土壤中球囊霉素含量的影响[J].农业环境科学学报,2016,35(7):1338-1343.

几种丛枝菌根真菌对菲污染土壤中球囊霉素含量的影响

Impacts of arbuscular mycorrhizal fungi on glomalin content in soils contaminated with phenanthrene

投稿时间：2016-04-03

DOI：10.11654/jaes.2016.07.016

中文关键词: 丛枝菌根真菌球囊霉素多环芳烃菌根修复

英文关键词: arbuscular mycorrhizal fungus glomalin polycyclic aromatic hydrocarbons rhizoremediation

基金项目:国家自然科学基金项目（21477056，31270574）；农业部公益性行业（农业）科研专项（201503107）

作者	单位	E-mail
杨振亚	江苏省环境科学研究院, 江苏省环境工程重点实验室, 南京 210036 南京农业大学土壤有机污染控制与修复研究所, 南京 210095
阙弘	南京农业大学土壤有机污染控制与修复研究所, 南京 210095
朱雪竹	南京农业大学土壤有机污染控制与修复研究所, 南京 210095
周紫艳	南京农业大学土壤有机污染控制与修复研究所, 南京 210095
陈爽	南京农业大学土壤有机污染控制与修复研究所, 南京 210095
凌婉婷	南京农业大学土壤有机污染控制与修复研究所, 南京 210095	lingwanting@njau.edu.cn

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中文摘要:

以菲为多环芳烃（PAHs）的代表，选用密色无梗囊霉（Acaulospora scrobculata，A.s）、摩西球囊霉（Glomus mosseae，G.m）、根内球囊霉（Glomus intraradices，G.i）、幼套球囊霉（Glomus etunicatum，G.e）和缩球囊霉（Glomus constrictum，G.c），宿主植物为紫花苜蓿（Medicago sativa L.），研究了接种丛枝菌根真菌（AMF）对菲污染土壤中球囊霉素相关土壤蛋白（GRSP，以下简称球囊霉素）含量的影响。供试时间（30～90 d）内，5种AMF均能对宿主植物根良好侵染，与紫花苜蓿建立共生关系，促进菲污染土壤中植物茎叶部和根部的生长；紫花苜蓿对5种AMF的菌根依赖性为108%～249%；接种AMF提高了土壤中GRSP的含量。90 d后接种G.i、G.e、G.m、G.c和A.s处理的土壤中总球囊霉素（T-GRSP）和易提取球囊霉素（EE-GRSP）含量分别达2.90～4.61、0.87～1.33 mg·g^-1，比不接种对照组（CK）分别高26.1%～100.0%和10.1%～68.4%；随着培养时间（30～90 d）延长，接种G.i的处理土壤中T-GRSP和EE-GRSP含量显著增大。接种同一AMF菌种（G.i）处理的土壤中T-GRSP和EE-GRSP含量与菲残留量间呈极显著负相关。

英文摘要:

Currently there is little information available about the responses of glomalin-related soil proteins (GRSP) to arbuscular mycor rhizal fungi (AMF) in soils contaminated with polycyclic aromatic hydrocarbons (PAHs). Here an investigation was conducted to examine the impacts of five AMFs on GRSP content in soils contaminated with phenanthrene as a representative PAH. The experimental AMFs were Acaulospora scrobculata (A.s), Glomus mosseae (G.m), Glomus intraradices (G.i), Glomus etunicatum (G.e) and Glomus constrictum (G.c). Host plant was alfalfa (Medicago sativa L.). These five AMFs had good colonization with alfalfa roots after 30~90 days. Inoculating AMFs enhanced GRSP content in phenanthrene-contaminated soils. Total extractable GRSP (T-GRSP) and easily extractable GRSP (EE-GRSP) were respectively 2.90~4.61 mg·g^-1 and 0.87~1.33 mg·g^-1 in soils inoculated with AMFs after 90-day cultivation, which were 26.1%~100.0% and 10.1%~68.4% higher than those in soils without AMF inoculation (CK). T-GRSP and EE-GRSP content in soils with AMF (G.i as a representative) was increased with cultivation time from 30 to 90 days, and significantly negatively correlated with residual concentrations of phenanthrene in soils.

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