钒胁迫下枸杞和芥菜幼苗蛋白的分子分布研究

侯明; 赵军平; 李明沅; 甘焕辉

文章摘要

侯明,赵军平,李明沅,甘焕辉.钒胁迫下枸杞和芥菜幼苗蛋白的分子分布研究[J].农业环境科学学报,2016,35(4):634-639.

钒胁迫下枸杞和芥菜幼苗蛋白的分子分布研究

Distribution of protein molecular in seedlings of Lycium barbarum and Brassica juncea under vanadiumstresses

投稿时间：2015-11-15

DOI：10.11654/jaes.2016.04.004

中文关键词: 钒胁迫枸杞芥菜分子分布蛋白质

英文关键词: vanadium stress Lycium barbarum Brassica juncea molecular distribution protein

基金项目:国家自然科学基金项目(41161076,41561077)

作者	单位
侯明	桂林理工大学化学与生物工程学院, 广西桂林 541004
赵军平	桂林理工大学化学与生物工程学院, 广西桂林 541004
李明沅	桂林理工大学化学与生物工程学院, 广西桂林 541004
甘焕辉	桂林理工大学化学与生物工程学院, 广西桂林 541004

摘要点击次数: 2666

全文下载次数: 3335

中文摘要:

通过G-75凝胶层析结合电泳分析,研究了不同V(吁)浓度胁迫下枸杞和芥菜的蛋白分子分布。经过凝胶层析后出现两个紫外吸收峰,第一峰主要集中在8号至15号管,第二峰集中在25号至33号管。对第一紫外吸收峰的电泳分析表明,V胁迫下植物蛋白的分子分布发生了变化:当V浓度为10 mg·L^-1时,枸杞根产生了分子量为58.6 KD蛋白,茎产生了分子量分别为64.5、46.9KD两种蛋白;当V浓度增大到20 mg·L^-1,叶产生了分子量为20 KD蛋白。随着V胁迫浓度增大,芥菜中分子量为33.9 KD和36KD两条蛋白条带逐渐变浅,而27.3 KD蛋白条带逐渐加深。枸杞幼苗第一洗脱峰蛋白中V含量随V胁迫浓度增大而增加,而芥菜叶在V≥10 mg·L^-1时V含量降低。这与不同植物蛋白结合V的能力有关,因枸杞幼苗蛋白结合V的能力较强,生成的植物螯合肽较多,故对V有较强的耐受能力。

英文摘要:

Protein molecular distribution in Lycium barbarum and Brassica juncea under different vanadium(V) stress levels was studied by G-75 gel chromatography and electrophoresis analysis. After gel chromatography, two UV peaks appeared, with the first peak occurring in No.8~No.15 tubes, and the second peak in No.25~No.33 tubes. Results of electrophoresis analysis from the first UV peak indicated that the molecular weight distribution changed under the V stresses. At 10 mg V·L^-1, Lycium barbarum produced a kind of protein with molecular weight of 58.6 KD in roots, while in stems two kinds of protein with molecular weight of 64.5 and 46.9 KD. At 20 mg V·L^-1, the leaves produced a kind of protein with molecular weight of 20 KD. With V stress levels increasing, two protein bands with 33.9 KD and 36 KD in Brassica juncea gradually changed to shallow, but that with 27.3 KD was deepened progressively. The V concentrations in the protein of the first elution peak in Lycium barbarum seedlings increased with increasing V stress levels, while the V concentrations in leaves of Brassica juncea decreased when V stress levels ≥10 mg·L^-1. This may be due to differences in V-binding capacity of different plant proteins. The V-binding ability of Lycium barbarum seedlings was greater than that of Brassica juncea, and thus more phytochelatins were generated, resulting in stronger tolerance of Lycium barbarum seedlings to V.

HTML 查看全文查看/发表评论下载PDF阅读器