| 晋海军,胡洪利,陈惠,张世熔,吴琦,孙蓉,唐自钟.DDRT-PCR技术分析籽粒苋镉胁迫下相关基因的差异表达[J].农业环境科学学报,2015,34(9):1659-1664. |
| DDRT-PCR技术分析籽粒苋镉胁迫下相关基因的差异表达 |
| Analysis of Differentially Expressed Genes of Amaranthus hypochondriacus Under Cadmium Stress by DDRT-PCR |
| 投稿时间：2015-05-24 |
| DOI：10.11654/jaes.2015.09.005 |
| 中文关键词: 籽粒苋 镉胁迫 DDRT-PCR技术 |
| 英文关键词: Amaranthus hypochondriacus cadmium stress DDRT-PCR |
| 基金项目: |
|
| 摘要点击次数: 3252 |
| 全文下载次数: 3086 |
| 中文摘要: |
| 以镉污染修复植物籽粒苋(Amaranthus hypochondriacus)的根、叶、果为实验材料,利用DDRT-PCR技术分析籽粒苋Cd胁迫下基因表达水平的变化。结果表明:回收差异片段获得镉处理诱导的4个差异表达序列(EST-1、EST-2、EST-3和EST-4),经Blastn比对,EST-1与土人参26S核糖体RNA基因部分序列(GB|HQ843466.1)一致性为99%,EST-2与虎耳草26S核糖体RNA大亚基基因部分序列(GB|AF036498.1)一致性为93%,EST-3与大肠杆菌菌株TW14359 DNA聚合酶Ⅲα亚基基因(ECs0186)完整CDS(GB|EU906049.1)一致性为97%,EST-4与米曲霉RIB40 β-葡萄糖苷酶基因(XM_001816779.2)一致性为99%;经Blastx比对,EST-1与假定蛋白MTR_5g051030[苜蓿](XP_003614386.1)氨基酸序列相似性为26%,EST-2与假定衰老相关蛋白[柏杉](GB|ACA30301.1)氨基酸序列一致性为98%,EST-3与DNA聚合酶Ⅲα亚基 [大肠杆菌OP50](ZP_06935700.1)氨基酸序列一致性为100%,EST-4与β-葡萄糖苷酶[黄曲霉NRRL3357](XP_002383240.1)氨基酸序列一致性为99%;4个差异表达基因通过调控蛋白质代谢、DNA复制和糖代谢等途径来应答镉胁迫。 |
| 英文摘要: |
| Amaranthus hypochondriacus has great potential for phytoremediation of cadmium(Cd) polluted soils. In this study, differential display reverse transcriptase polymerase chain reaction(DDRT-PCR) was used to screen differentially expressed genes in the roots, leaves and fruits of A. hypochondriacus under Cd stress. The novel bands were recovered and four differentially expressed cDNA fragments[EST(Expressed sequence tags)-1, EST-2, EST-3 and EST-4] were obtained. All ESTs had homology with known cDNA clones in the Genbank databases. The EST-1 was similar to partial sequences of Talinum paniculatum 26S ribosomal RNA gene(GB|HQ843466.1)with an identity of 99%, while the EST-2 showed similarity to partial sequences of Saxifraga mertensiana large subunit 26S ribosomal RNA gene(GB|AF036498.1) with an identity of 93%. The EST-3 was similar to complete cds of Escherichia coli strain TW14359 DNA polymerase Ⅲ alpha subunit(ECs0186) gene(GB|EU906049.1) and their identity was 97%. The EST-4 was similar to Aspergillus oryzae RIB40 beta-glucosidase A(XM_001816779.2) and the identity was 99%. After BlastX comparison, EST-1 was similar to hypothetical protein MTR_5g051030 [Medicago truncatula](XP_003614386.1) with an identity of 26%, EST-2 similar to putative senescence-associated protein[Cupressus sempervirens](GB|ACA30301.1) with an identity of 98%, EST-3 similar to DNA polymerase Ⅲ subunit alpha[Escherichia coli OP50](ZP_06935700.1) with an identity of 100%, and EST-4 similar to beta-glucosidase[Aspergillus flavus NRRL3357](XP_002383240.1) with an identity of 99%. These four differentially expressed genes were responsive to cadmium stress through regulating protein metabolism, DNA replication and glucose metabolism. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
