| 陈志鹏,魏源,上官宇先,侯红,曾清如.丛枝菌根真菌对紫花苜蓿锑积累和抗氧化活性的影响[J].农业环境科学学报,2015,34(6):1053-1059. |
| 丛枝菌根真菌对紫花苜蓿锑积累和抗氧化活性的影响 |
| Effect of Arbuscular Mycorrhizal Fungi on Antimony Uptake and Antioxidative Responses of Medicago Sativa in Antimony Polluted Soil |
| 投稿时间：2010-02-05 |
| DOI：10.11654/jaes.2015.06.006 |
| 中文关键词: 丛枝菌根真菌 锑 抗氧化酶 紫花苜蓿 |
| 英文关键词: arbuscular mycorrhizal fungi antimony antioxidant enzyme system Medicago Sativa |
| 基金项目:国家自然科学基金(41271338, 41303066);中国博士后面上基金(2013M530685) |
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| 中文摘要: |
| 利用温室盆栽试验, 研究了不同锑浓度(0、500、1000 mg·kg-1)土壤处理下混合接种丛枝菌根(Arbuscular mycorrhizal,AM)真菌(Funneliformis mosseas和Glomus versiforme)后对苜蓿(Medicago Sativa)的锑累积量、生物量和抗氧化酶活性的影响。结果表明:接种AM真菌显着促进了苜蓿对锑的积累。在土壤锑浓度为500 mg·kg-1时, 接种AM真菌地上和地下部分锑含量分别增加了71%和46%;在锑浓度为1000 mg·kg-1时, 地上和地下部分锑含量增加了37%和34%.在土壤锑胁迫条件下, 接种AM真菌能显着提高苜蓿地上部分对磷的吸收, 这可能是苜蓿生物量增加的原因。同时, 接种处理降低了苜蓿中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性。在锑浓度为500 mg·kg-1,接种AM真菌显着降低了苜蓿SOD和POD活性;在锑浓度为1000 mg·kg-1时, 接种AM真菌显着降低SOD活性。 |
| 英文摘要: |
| Under greenhouse pot experiment conditions, mixed arbuscular mycorrhizal(AM) fungi(Funneliformis mosseas and Glomus versiforme) were inoculated on Medicago Sativa(M. sativa), and the effects on the biomass, accumulation of antimony(Sb) and antioxidant enzyme system of the plant were investigated. The experiments were conducted using soil treated with Sb at 0, 500 and 1000 mg·kg-1. The results indicated that the inoculation greatly promoted the accumulation of Sb in M. sativa. Compared to the non-inoculated treatment, the Sb concentrations in the shoots and roots of M. sativa increased 71% and 46% under 500 mg·kg-1 Sb-treated soil, and 37%, 34% under 1000 mg·kg-1 Sb-treated soil, respectively. Inoculation also significantly enhanced the adsorption of phosphorus(P) in M. sativa, which may induced higher biomass of the plant. Meanwhile, Inoculation reduced the peroxidase(POD), superoxide dismutase(SOD) and catalase (CAT) activities in M. sativa. In conclusion, M. sativa inoculated with arbuscular mycorrhizal fungi were less sensitive to Sb stress than uninoculated plants. This results suggested that AMF play an important role in the uptake of Sb by plant, and also its resistance to Sb. |
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