| 沈伟航,朱能武,王华金,党志,陈小强.石油污染土壤生物修复过程中毒性的植物指示[J].农业环境科学学报,2015,34(1):22-28. |
| 石油污染土壤生物修复过程中毒性的植物指示 |
| Plant Bio-indicators of Eco-toxicity of Oil-contaminated Soil During Bioremediation |
| 投稿时间：2014-07-26 |
| DOI：10.11654/jaes.2015.01.004 |
| 中文关键词: 土壤 石油生物修复 生物修复 高等植物 生物指示 |
| 英文关键词: soil crude oil contamination bioremediation higher plants bio-indicator |
| 基金项目:广东省自然科学基金团队项目(9351064101000001);教育部新世纪优秀人才支持计划项目(NCET-11-0166) |
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| 中文摘要: |
| 为探究石油污染土壤生物修复过程中土壤生态毒性的变化规律并完成指示生物和指标的筛选,采用前期筛选和分离的三株对石油烃有良好降解效果的降解菌构建了混合菌体系,开展了石油污染土壤模拟生物修复实验,考察了不同修复时期小麦和萝卜种子的发芽率和幼苗生长发育状况(株高、地上部鲜重、根鲜重、根干重)及叶片的malonaldehyde(MDA)含量、光合色素含量(叶绿素a和类胡萝卜素)、superoxide dismutase(SOD)活性的变化.结果表明:微生物修复8 d和16 d的土壤,其生态毒性较强;前期土壤的生态毒性有所上升,随后有所降低,在整个修复时期土壤均存在一定的生态毒性;不同的指示植物、指示指标对石油污染的响应存在一定的差异.幼苗生长发育指标(株高、地上部鲜重、根鲜重)以及叶片SOD活性、MDA含量等指标均能较好指示整个石油污染土壤微生物修复过程中的生态毒性,可作为石油污染土壤微生物修复效果的指示生物和指标. |
| 英文摘要: |
| Seed germination, seedling growth and leaf biochemical parameters of plant could be used to comprehensively reflect soil health. In order to screen plant bio-indicators of oil-contaminated soil and to explore the change pattern of its eco-toxicity during bioremediation, a bioremediation experiment was conducted. Crude oil degrading bacterial consortium were built with three strains isolated from oil-contaminated soil. Seed germination, seedling growth, and leaf photosynthetic pigment content, MDA(malonaldehyde) content and SOD(superoxide dismutase) activities of wheat and radish were measured. During the bioremediation process, soil eco-toxicity was the greatest on the 16th day and gradually dropped down to a normal level thereafter. Soil eco-toxicity was different for different plant bio-indicators applied. The present findings suggest that seed germination, seedling growth, and leaf photosynthetic pigment content, MDA and SOD activities could be used as potential indicators of the effectiveness of microbial remediation of oil-contaminated soils. |
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