崔茂金,黄明贤,邓月娥.稻秆盐酸预处理产还原糖的工艺优化[J].农业环境科学学报,2014,33(3):613-616. |
稻秆盐酸预处理产还原糖的工艺优化 |
Optimization of Conditions for Producing Reducing Sugar from Rice Straw Pretreated with Hydrochloric Acid |
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DOI:10.11654/jaes.2014.03.031 |
中文关键词: 稻秆 HCl预处理 总还原糖 正交实验 Box-Behnken设计 |
英文关键词: rice straw HCl pretreatment total reducing sugar orthogonal design Box-Behnken design |
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中文摘要: |
为了提高稻秆的糖化率,采用正交实验和响应曲面法优化稻秆盐酸(HCl)预处理条件。首先通过正交实验研究了预处理温度、HCl浓度、预处理时间和稻秆浓度四个因素对总还原糖的影响;然后在正交实验的基础上采用Box-Behnken设计对HCl预处理条件进行进一步优化。研究结果表明:HCl预处理条件主次因素为预处理温度、预处理时间、HCl浓度和稻秆浓度;稻秆HCl预处理最佳条件是预处理温度133.80 ℃、HCl浓度7.28%(W/V)和预处理时间51.12 min,此时总还原糖量为549.42 mg·g-1,是未预处理稻秆总还原糖浓度的6.01倍,这说明HCl预处理对于提高稻秆的糖化率是有必要的。 |
英文摘要: |
Producing bioenergy from agricultural wastes(e.g. rice straw) is a way to kill two birds with one stone by recycling agricultural wastes and obtaining new energy. To improve saccharification efficiency of rice straw, orthogonal design and response surface methodology were used to optimize temperature, time and concentration of hydrochloric acid(HCl) pretreatment and rice straw rates. Box-Behnken design was then employed to further optimize the HCl pretreatment conditions. The factors influencing production of total reducing sugar were in order of temperature>HCl concentration>time>rice straw rates. The optimal conditions for HCl pretreatment of rice straw were temperature of 133.80 ℃, HCl concentration of 7.28%(W/V) and time of 51.12 min. The maximum concentration of total reducing sugar was 549.42 mg·g-1 under the optimal conditions, which was 6.01 times of that from raw materials. The results indicate that HCl pretreatment is necessary for higher saccharification of rice straw. |
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