| 秦 莉,高茹英,徐亚平,胡 菊.堆肥中高效降解纤维素及金霉素和土霉素的复合菌系的构建[J].农业环境科学学报,2014,33(3):465-470. |
| 堆肥中高效降解纤维素及金霉素和土霉素的复合菌系的构建 |
| Construction of a High-Efficiency Complex Microbial System to Degrade Cellulose and Chlortetracycline and Oxytetracycline in Compost |
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| DOI：10.11654/jaes.2014.03.009 |
| 中文关键词: 复合菌系 堆肥 纤维素 抗生素 降解菌 金霉素 土霉素 |
| 英文关键词: complex microbial system compost cellulose antibiotic degradation bacteria chlortetracycline oxytetracycline |
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| 摘要点击次数: 2964 |
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| 中文摘要: |
| 为构建有效降解纤维素和抗生素类兽药（金霉素和土霉素）复合功能的微生物菌系，以4种高温期堆肥样品为菌种来源，经多代优化组合，最终筛选驯化出能有效降解纤维素及金霉素和土霉素的一组复合微生物菌系，该菌系对pH的适应性较强。通过对复合菌系在传代（20、25代和30代）过程中的稳定性研究，3代发酵液的pH变化几乎没有差异：在接种后24 h内，发酵液pH由开始时的8.5迅速下降到7.0以下；接种后48 h内，pH稳定在7.0左右；在接种后72 h，pH恢复到8.0左右，即均呈现先降后升的趋势；3代复合菌系在第3 d的滤纸分解率均超过90%；在发酵结束时，金霉素和土霉素降解率均超过50%，且3代之间差异极小。复合菌系在分解纤维素及金霉素和土霉素方面具备稳定能力。 |
| 英文摘要: |
| Composting is an effective way to dispose animal manures. However, cellulose and antibiotics in the manures often impact the maturity speeds of composts. Here we built a complex microbial system capable of degrading cellulose and chlortetracycline（CTC） and oxytetracycline（OTC） efficiently in 4 types of thermophilic compost samples through several generations of portfolio optimization. This complex microbial system adapted a wider range of pH, and had strong stability. For the third generation, its medium pH reduced to 7.0 from initial 8.5 within 24 h of inoculation, stayed at 7.0 for 48 h and then raised to 8.0 after 72 h. The microbial system degraded filter paper by more than 90% in 3 days, and CTC and OTC by more than 50% in 7 days of inoculation. The results indicate that the constructed complex microbial system has stable capability of degrading cellulose and antibiotics（CTC and OTC）. |
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