| .异恶草酮对蚯蚓抗氧化酶活性及DNA损伤的研究[J].农业环境科学学报,2013,32(5):. |
| 异恶草酮对蚯蚓抗氧化酶活性及DNA损伤的研究 |
| Effects of Clomazone on Antioxidative Enzymes Activity and DNA Damage of Earthworm（Eisenia foetida） |
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| DOI：10.11654/jaes.2013.05.007 |
| 中文关键词: 蚯蚓 异恶草酮 抗氧化酶 DNA损伤 |
| 英文关键词: earthworm clomazone activity of antioxidative enzymes DNA damage |
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| 摘要点击次数: 2567 |
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| 中文摘要: |
| 采用人工土壤法研究了染毒14 d后不同浓度（0、10、20、40、80 mg·kg-1）异恶草酮对赤子爱胜蚓（Eisenia foetida）超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）活性，总抗氧化能力（T-AOC）和体腔细胞DNA损伤的影响。结果表明：随着暴露浓度的增加，SOD活性呈现先上升后逐渐下降的趋势，低浓度（10、20 mg·kg-1）能显著诱导SOD酶活性（P<0.01，P<0.05），诱导率分别为19.56%、13.03%。CAT活性在较低浓度时没有显著性变化，当浓度达到40、80 mg·kg-1时出现显著性诱导（P<0.01），诱导率分别为43.42%、68.08%。T-AOC在整个胁迫阶段呈现先降低后上升的趋势，最终趋于正常水平，20 mg·kg-1暴露组T-AOC含量最低（5.74 U·mg-1 pro），比对照组减少了44.54%。GSH-Px酶活性在整个暴露阶段处于抑制状态。不同浓度的异恶草酮均能引起蚯蚓体腔细胞DNA损伤，暴露浓度与尾部DNA含量、Olive尾距和尾长具有良好的剂量-效应关系（P<0.01）。上述结果表明抗氧化酶活性及DNA损伤可作为异恶草酮影响的生物标志物，异恶草酮对土壤动物蚯蚓具有毒性作用，对土壤环境具有潜在的生态风险。 |
| 英文摘要: |
| Effects of clomazone at different concentration（0 mg·kg-1, 10 mg·kg-1, 20 mg·kg-1, 40 mg·kg-1, 80 mg·kg-1） on activity of antioxidative enzymes of SOD, CAT, GSH-Px, T-AOC and DNA damage in earthworm（Eisenia foetida） were studied with an artificial soil test in 14 days of exposure. During the exposure period, there was a trend on the SOD activity by an initial significant increase（P<0.05） and then following a gradual decrease. When the earthworms were exposed to 10 mg·kg-1 and 20 mg·kg-1 of clomazone, the activity of SOD increased significantly with induced rate of 19.56% and 13.03%, respectively. The activity of CAT had no significant change at the low concentrations（10 mg·kg-1, 20 mg·kg-1）, and was induced at the higher concentrations（40 mg·kg-1, 80 mg·kg-1） with induced rate of 43.42% and 68.08%, respectively. The content of T-AOC was inhibited first but induced, then recovering to the level of control gradually. The content of T-AOC exposed to the concentration of 20 mg·kg-1 decreased significantly with inhibition rate of 44.54%. The activity of GSH-Px was inhibited significantly during the period of exposure. The results showed that clomazone could induce DNA damage of earthworm coelomocytes at different exposure concentration. The significant dose-effect relationships were found among clomazone concentration and tail DNA percent, OTM as well as tail length（P<0.01）. The activity of antioxidative enzymes and DNA damage could be as biomarkers to indicate the direct toxic effect of clomazone on soil animals. The results indicated that clomazone had a potential ecological risk on soil environment. |
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