| .松花湖水体中不同空间分布的细菌群落结构分析[J].农业环境科学学报,2013,32(4):. |
| 松花湖水体中不同空间分布的细菌群落结构分析 |
| Bacterial Community Structure in Different Spatial Distribution of Songhua Lake |
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| DOI：10.11654/jaes.2013.04.016 |
| 中文关键词: 微生物多样性 PCR-DGGE 环境因子 空间分布 |
| 英文关键词: microbial diversity PCR-DGGE environmental factors spatial distribution |
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| 中文摘要: |
| 为了研究松花湖水体中微生物群落结构的多样性，采用聚合酶链式反应-变性凝胶梯度电泳（PCR-DGGE）对水体垂向空间分布的细菌进行了多样性分析。把每一个条带看作一个分类操作单位（OUT），表明水体细菌的微生物群落结构随着湖泊深度变化呈现显著的差异，其中S1和S2位点微生物群落单元（OUT）数高于其他位点。UPGMA聚类分析DGGE指纹图谱表明，水体不同深度的微生物群落结构之间相似性和动态性存在明显差异。微生物群落单元数与环境因子之间响应关系的典型对应分析（CCA）证实，水深（SH）、pH、温度（Wt）和溶解氧（DO）等环境因子与微生物群落多样性指标之间存在显著相关性，水深（SH）、温度（Wt）的变化是微生物群落结构变化的主要原因。 |
| 英文摘要: |
| In order to study the diversity of microbial community structure in Songhua Lake，PCR-DGGE（Polymerase chain reaction-denaturing gradient gel electrophoresis） technology is employed to study the bacterial community structure in the different spatial distribution. Each strip as a operational taxonomic unit（OUT）, suggested that bacterial microbial communities with water depth change will have a significant difference, microbial community unit（OUT） numbers higher than other sites in S1 and S2. The Unweighted pair group method with arithmetic averages（UPGMA） dendrogram of sample basis on DGGE fingerprints showed water microbial community diversity presents significant spatial difference, there have similar and dynamic in the different depths of water. Canonical correspondence analysis（CCA） was applied to further investigate the relationships between OTU composition and the environmental factors. The first two CCA ordination axes suggested that the bacterial community composition was primarily correlated with the variables of depth of water（SH）, temperature（Wt），and dissolved oxygen（DO）. The change of depth of water（SH） and temperature（Wt） were the main reason for the change of microbial community structure. |
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