| .转基因大豆对根际土壤微生物群落功能多样性的影响[J].农业环境科学学报,2013,32(2):. |
| 转基因大豆对根际土壤微生物群落功能多样性的影响 |
| Effects of Transgenic Soybeans on the Microbial Functional Diversity in the Rhizosphere |
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| DOI：10.11654/jaes.2013.02.012 |
| 中文关键词: 转基因大豆 土壤微生物 功能多样性 Biolog 主成分分析 |
| 英文关键词: transgenic soybean soil microbe functional diversity Biolog principal component analysis |
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| 中文摘要: |
| 采用大田试验结合Biolog技术比较分析了种植转基因大豆PAT与其亲本PAT1、转基因大豆ALS与其亲本ALS1及当地主栽大豆中黄13对根际土壤微生物群落功能多样性的影响。结果表明，由平均颜色变化率（AWCD）反映的土壤微生物活性呈现以下变化规律：在整个温育过程中，两种转基因大豆根际土壤微生物活性均高于相应亲本，当地品种中黄13根际土壤微生物活性介于转基因大豆和非转基因亲本大豆之间；两种转基因大豆根际土壤微生物群落物种均一度（J）和优势度指数（D）较对应亲本均无显著差异（P>0.05），转基因大豆PAT根际土壤微生物群落香农-维纳指数（H）与亲本相比差异不显著（P>0.05)，而转基因大豆ALS根际土壤微生物群落香农-维纳指数（H）显著高于其亲本ALS1（P<0.05）。主成分分析结果表明，转基因大豆PAT、亲本PAT1、非转基因亲本大豆ALS1以及中黄13土壤微生物群落碳源利用类型相似，仅转基因大豆ALS土壤微生物碳源利用类型表现出差异。对不同碳源的分析结果表明，土壤微生物利用的主要碳源为糖类、氨基酸类、羧酸类和聚合物。 |
| 英文摘要: |
| Transgenic crops are grown on an increasingly large scale throughout the world, and their increased use has been accompanied by public concern about their potential ecological and environmental impacts. The persistence and interaction of proteins derived from transgenic crops in soil and their effects on soil microbial are essential information when investigating their effects within the soil ecosystem. The functional diversity of microbial communities in the rhizosphere of transgenic soybean PAT and its parental isoline PAT1, transgenic soybean ALS and its parental isoline ALS1, and local major soybean zhonghuang13 was analyzed under field experiment by Biolog-ECO technique. Soil samples at the depth of 0~20 cm were collected in September 2011 at mature stage. The functional diversity of the soil microbial communities from the different samples was further analyzed by different techniques, such as statistical analysis and diversity index evaluation of the soil microbia. The results showed that the average well color development（AWCD） which directly reflected the microbial activities of changed in the following variation：All the treatments exhibited the elevation of AWCD during the first 48 hours of incubation, but the differences among the treatments were obvious. Both transgenic soybean PAT and ALS caused high increase of the AWCD while the corresponding non-transgenic parental isoline and local major soybean zhonghuang13 had less affection on the AWCD. During the whole incubation process, the soil microbial activity of transgenic soybean PAT and ALS was higher than the corresponding non-transgenic parental isoline, and the local major soybean zhonghuang 13 was between the transgenic soybeans and non-transgenic parental isolines. The species evenness（J）and dominance index（D） of the soil microbial communities of transgenic soybeans showed no significant difference（P>0.05） compared with the non-transgenic parental isolines, The Shannon-Wiener index（H） of the soil microbial communities of the transgenic soybean ALS was higher than the its parental isoline ALS1, with no difference between the transgenic soybean PAT and its parental isoline PAT1. The principal component analysis（PCA） indicated that the variance contribution rate of PC1 and PC2 were 37.28% and 17.19%, respectively. The correlation coefficients between main substrates and PC1 or PC2 indicated that the main carbon sources for soil microbes were carbohydrates, amino acids, carboxylic acids and polymers in all treatments. The carbon source utilization mode and metabolic function of the microbial communities were similar among the transgenic soybean PAT, the parental isoline PAT1, the parental isoline ALS1 and local major soybean zhonghuang13, unless the transgenic soybean ALS． |
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