|
| 增氧方式对水芹菜-微生物联合作用处理冬季养殖废水的影响 |
| Effect of different aeration methods on swine wastewater treated by Oenanthe javanica combined with microbes in winter |
| Received:June 29, 2019 |
| DOI：10.13254/j.jare.2019.0345 |
| 中文关键词: 水芹菜，污水净化，群落结构，高通量测序 |
| 英文关键词: Oenanthe javanica, wastewater purification, microbial community, Illumina sequencing |
| 基金项目:温州市科技计划项目（W20170009） |
| Author Name | Affiliation | E-mail | | LIU Fang-jian | College of Life and Environmental Science, Wenzhou University, Wenzhou 325000, China | | | YANG Hai-long | College of Life and Environmental Science, Wenzhou University, Wenzhou 325000, China | | | CHEN Xiao-lu | College of Life and Environmental Science, Wenzhou University, Wenzhou 325000, China | | | LI Lin-qiu | College of Life and Environmental Science, Wenzhou University, Wenzhou 325000, China | | | ZHOU Hua-bin | College of Life and Environmental Science, Wenzhou University, Wenzhou 325000, China | zhb@wzu.edu.cn |
|
| Hits: 1381 |
| Download times: 1955 |
| 中文摘要: |
| 为探究不同增氧方式对水芹菜-微生物联合作用处理养殖废水消化液及微生物群落结构变化的影响，设置循环和曝气两组试验，以静置处理作为对照，共运行35 d，间隔7 d取样，测定常规水质指标，在试验开始和结束时分别测定水芹菜生长情况，并采用高通量测序技术对微生物群落结构进行分析。结果显示，经过35 d的处理，静置组、曝气组和循环组的DO（溶解氧）分别达到0.60、10.38 mg·L-1和10.85 mg·L-1；静置培养的水芹菜长势最好，相对生长速率为0.03 g·g-1·d-1；三种处理方式对水质的净化效果差异显著，循环组的水质净化效果最好，COD（化学需氧量）、NH3-N（氨氮）和TN（总氮）的去除率分别达89.89%、69.40%和77.65%；黄杆菌属（Flavobacterium）的相对丰度从43.21%下降到0.16%，克里斯滕森菌属（Christensenellaceae）和瘤胃球菌属（Ruminococcaceae）的相对丰度则大幅增加。水芹菜在冬季于养殖废水消化液中可以正常生长，曝气和循环处理可以明显提高净化效率；黄杆菌属和假单胞菌属（Pseudononas）是脱氮的功能菌属，梭菌纲（Clostridia）的大量繁殖有利于COD的去除。 |
| 英文摘要: |
| The aim of this research is to study the effect of different aeration methods on swine wastewater treated by Oenanthe javanica combined with microbes, and the change in the microbial community. Experiments were conducted by circulation, aeration, and standing (control) groups, and a sample was taken at 7-day intervals during the treatment period of 35 days. The water indices were analyzed by national standard methods. The microbial community characteristics were analyzed using a high-throughput sequencing technology (Illumina MiSeq sequencer). The dissolved oxygen values in the control, aeration, and circulation groups were 0.60, 10.38 mg·L-1 and 10.85 mg·L-1, respectively. The best growth of Oenanthe javanica was observed in the control group, where a relative growth rate of 0.03 g·g-1·d-1 was achieved. The water purification efficiency of the three treatments was significantly different, in this case, the circulation group showed superior results with the removal rates of chemical oxygen demand (COD), ammonium nitrogen, and total nitrogen at 89.89%, 69.40%, and 77.65%, respectively. The relative abundance of Flavobacterium decreased from 43.21% to 0.16%, while that of Christensenellaceae and Ruminococcaceae increased significantly. Oenanthe javanica grew well in the treated swine wastewater during winter. The purification efficiency can be obviously improved by supplementing oxygen through circulation or aeration. After 35 days of treatment, the microbial diversity in the water increased; Moreover, Flavobacteria and Pseudomonas were identified as the functional bacteria of denitrification. The proliferation of Clostridiales is also beneficial for the removal of COD. |
| HTML
View Full Text
View/Add Comment Download reader |
| Close |
|
|
|
