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| 三种抗生素与铜复合污染对土壤过氧化氢酶活性的影响 |
| Effect of combined pollution of three antibiotics and Cu on soil catalase activity |
| Received:December 18, 2018 |
| DOI：10.13254/j.jare.2018.0369 |
| 中文关键词: 恩诺沙星，土霉素，磺胺二甲嘧啶，铜，土壤过氧化氢酶 |
| 英文关键词: enrofloxacin, oxytetracycline, sulfamethazine, Cu, soil catalase |
| 基金项目:国家科技重大专项（2016YFD0201203）；山东省自然科学基金项目（ZR2016JL029，JQ201711）；泰山学者工程专项 |
| Author Name | Affiliation | E-mail | | ZHANG Wen-jie | College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Tai'an 271018, China | | | YANG Li-li | College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Tai'an 271018, China | | | WANG Jin-hua | College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Tai'an 271018, China | wjh@sdau.edu.cn | | ZHU Lu-sheng | College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Tai'an 271018, China | | | WANG Jun | College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Tai'an 271018, China | | | MAO Shu-shuai | College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Tai'an 271018, China | |
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| 中文摘要: |
| 为分析抗生素与重金属单一及复合污染对土壤过氧化氢酶活性的影响，以三种兽用抗生素恩诺沙星（Enrofloxacin，ENR）、土霉素（Oxytetracycline，OTC）、磺胺二甲嘧啶（Sulfamethazine，SM2）和重金属铜（Cu）为目标污染物，采用高锰酸钾滴定法，研究其对土壤中过氧化氢酶活性的影响。结果表明，单一污染下，中低浓度的ENR各处理对酶活性有一定激活作用，最大激活率为5.52%；OTC处理组随着污染物浓度的增加，对酶活性的抑制作用增大，抑制率最大为8.36%；SM2处理初期，中低浓度处理组对酶有激活作用，最大激活率为7.46%，处理21 d，高浓度处理组对酶活性有显著促进作用，激活率为1.71%。Cu在第28 d时对过氧化氢酶活性表现为抑制。复合污染下，ENR与Cu复合污染组对过氧化氢酶活性的影响随污染浓度增加先抑制后激活；OTC与Cu处理第7 d，各浓度组与对照差异均不显著，14 d后，高浓度组产生抑制作用，抑制率最高为3.58%；SM2与Cu高浓度处理组在第7 d对土壤过氧化氢酶活性表现为明显激活作用，激活率高达10.00%，14 d后转为抑制作用，28 d时抑制率最高为4.49%。研究表明，单一污染下，抗生素对土壤过氧化氢酶的影响与其类型、暴露浓度以及暴露时间有关。复合污染下，抗生素和Cu的交互作用存在差异，表现为拮抗和协同两种作用，不仅与抗生素种类、复合污染浓度比例、暴露时间有关，还与抗生素和Cu配位反应产生复合物的比例有关。 |
| 英文摘要: |
| To analyze the single and joint toxicity of antibiotics and heavy metals on the soil catalase ecosystem, this study regarded three kinds of antibiotics, enrofloxacin(ENR), oxytetracycline(OTC), and sulfamethazine(SM2), and Cu as target contaminants. Potassium permanganate titration was used to study the effects of these contaminants on the activity of catalase, which is an important indicator of soil pollution. The results showed that the treatment of medium and low concentrations of ENR had a certain activation effect on enzyme activity, and the maximum activation rate was 5.52%. With the increase of exposure concentration, the inhibitory effect of OTC on enzyme activity increased, and the inhibition rate increased to 8.36%. At the initial stage of SM2 treatment, the medium and low concentration treatment group activated the enzyme, and the maximum activation rate reached 7.46%. After treatment for 21 d, The high concentration treatment group activated the enzyme on day 21, and the activation rate was 1.71%. Cu inhibited catalase activity on day 28. The effect of ENR+Cu treatment on catalase activity was firstly inhibited and then activated with the increase of pollution concentration; on day 7 of the OTC+Cu treatment, all the concentration groups had no significant difference with the control, and after 14 d, the high concentration group inhibited soil catalase activity with the highest inhibition rate of 3.58%. The SM2+Cu treatment groups had an inhibition effect following the activation effect, with an activation rate of 10.00% on day 7 and an inhibition rate of 4.49% on day 28. During the entire experimental cycle, the ENR+Cu and SM2+Cu treatment groups were affected by concentration and time; antagonism dominated the OTC+Cu composite pollution effects. All the findings in this study demonstrated that in the case of a single pollutant, the effect of antibiotics on soil catalase was related to its type, exposure concentration, and exposure time. With a combination of pollutants, the interaction between antibiotics and Cu varied, and was characterized by antagonistic and synergistic effects, which were related to the type of antibiotic, ratio of combined pollution concentration and exposure time, and the ratio of antibiotic and Cu coordination reactions to produce complexes. |
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