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| 减肥条件下生物质炭施用对水稻田土壤细菌多样性的影响 |
| Effects of biochar addition on microbial diversity of paddy soil under fertilizer reduction |
| 投稿时间：2020-06-18 修订日期：2020-07-17 |
| DOI： |
| 中文关键词: 生物质炭 水稻田土壤 根际土壤 细菌多样性 高通量测序 |
| 英文关键词: biochar paddy soil microbial diversity rhizosphere soil high throughput sequencing |
| 基金项目:国家自然科学基金( 51508366) ; 江苏省自然科学(SBK2020022850)；苏州昆山市科技计划项目(KN1807)；江苏水处理技术与材料协同创新中心预研项目(XTCXSZ2019-3)Project supported：National Natural Science Foundation of China (No.51508366)；Natural Science Foundation of Jiangsu Province (No. SBK2020022850)；Kunshan Municipal Science and Technology Project(KN1807)；Pre-research Fund of Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment (XTCXSZ2019-3) |
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| 中文摘要: |
| 为了研究肥料减施和水稻秸秆生物质炭添加对水稻田土壤细菌多样性的影响，本文采用Miseq高通量测序分析技术，对不同施肥量（100%，90%和80%常规施肥）和添加水稻秸秆生物质炭（1%）的水稻田非根际和根际土壤进行了细菌多样性分析，发现生物质炭输入对非根际和根际土壤细菌多样性造成影响，且根际微生物细菌多样性更加丰富。按门水平来看，最主要是变形菌门，占34.85-47.57%，生物质炭添加降低了变形菌门在非根际土中的丰度，而促进其在根际土中富集，肥料减施对变形菌门丰度的影响刚好相反，且减施肥料越多对变形菌门影响越大；酸杆菌门次之，占10.48-19.42%，变化趋势与变形菌门相左。unclassified Burkholderiaceae，unclassified_Subgroup 6、uncultured bacterium 126等属在土壤中占比较高。典范对应分析(CCA)结果表明，优势菌群丰度与采样位置、肥料添加、生物质炭添加等环境因素存在一定的相关性。 |
| 英文摘要: |
| In order to study the effects of rice straw biochar addition on microbial diversity of paddy soil under fertilizer reduction, the Miseq high-throughput sequencing analysis technology was used to analyze the microbial diversity of rhizosphere and bulk soil under different fertilization application (100%, 90% and 80% of conventional fertilization) and rice straw biochar addition (1%). The result indicated that rice straw biochar addition could influence the microbial diversity of rhizosphere and bulk soil. However, the microbial diversity of rhizosphere soil was more abundant than bulk soil. The Proteobacteria was the main bacteria at a phylum level which accounting for 34.85-47.57%. Biochar addition could reduce the Proteobacteria enrichment in the bulk soil and promote it in the rhizosphere soil. However, the reduction of fertilizer was opposite effect on the abundance of Proteobacteria, while the greater influence of proteobacteria was presented with more fertilizer reduction. In addition, the Acidobacteria accounting for 10.48-19.42% was followed by Proteobacteria, which was opposite to the change rule of Proteobacteria. Unclassified Burkholderiaceae, unclassified_Subgroup 6, uncultured bacterium 126 was account for a relatively high proportion in rhizosphere and bulk soil at a genus level. The results of Canonical Correspondence Analysis (CCA) showed that the abundance of dominant bacteria was related to environmental factors such as sampling location, fertilizer addition and biochar addition, respectively. |
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