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| 一株烟草肠杆菌(Enterobacter tabaci)S4菌株的鉴定及其效果测定 |
| Identification and evaluation of Enterobacter tabaci strain S4 |
| 投稿时间：2019-09-23 修订日期：2019-12-23 |
| DOI： |
| 中文关键词: 植物生长调节剂 分子鉴定 生物测定 肠杆菌 番茄 |
| 英文关键词: Plant growth regulator Molecular identification Bioassay Enterobacter tabaci Tomato |
| 基金项目:丹江口水源涵养区绿色高效农业技术创新集成与示范（CAAS-XTCT2016015）；基于海洋微生物功能分子的绿色生物制剂研制与应用示范（桂科AA18242026）；广西科技基地和人才专项（桂科AD18126005） |
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| 中文摘要: |
| 【目的】为筛选出一株具有微生物源植物调节剂功能的细菌，并探讨其潜在的生物功能。【方法】本研究利用平板涂布法以及16S rRNA鉴定技术对菌株进行分离、鉴定；同时使用该菌株发酵液对番茄的种子和幼苗分别进行浸种和灌根处理，并进行农艺指标测定。【结果】结果表明：经鉴定该菌株为烟草肠杆菌，其发酵液对番茄种子的萌发、幼苗的生长具有显著的促进作用，其中发酵液浓度为1×104 cfu·mL-1时促生效果最好，与对照相比，根长、株高、干重和鲜重分别增加63.42%、46.17%、150.00%和144.83%。【结论】研究结果可为该菌株开发为微生物肥料或微生物源植物生长调节剂提供技术支持。 |
| 英文摘要: |
| To screen a microbial strain of bacteria with the function of plant promotion and explore its potential biological functions, the strains were isolated and identified by plate coating method and 16S rRNA identification technology in this study. The seed and seedling of tomato were treatment by using the fermentation broth and the biological agronomic indexes were determined. The results showed that the strain was identified as Enterobacter tabaci, which is the fermentation broth significantly promoted the germination and seedling growth of tomato seeds. Fermentation broth at a concentration of 1 × 104 cfu·mL-1 had the best growth promoting effect. Compared with the control, the root length, plant height, dry weight and fresh weight increased by 63.42%, 46.17%, 150.00% and 144.83%, respectively. The results can provide technical support for the development of the strain as microbial fertilizer or microbial source plant growth promotion. |
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