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| 石油污染土壤中正十六烷降解菌的效果研究 |
| Degradation Capability of n-hexadecane Degrading Bacteria from Petroleum Contaminated Soils |
| 投稿时间：2017-01-11 |
| DOI：10.13254/j.jare.2017.0014 |
| 中文关键词: 石油污染土壤，正十六烷，降解菌，环境因子，降解率 |
| 英文关键词: petroleum contaminated soil, n-hexadecane, degrading bacteria, environmental factors, degradation rate |
| 基金项目:“韩国量子能公司农业用颗粒（丸）粉末及使用方法在中国农业生产中实用效果测试计划”[开（2015）1]；国家水体污染控制与治理科技重大专项（2014ZX07101-012-001） |
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| 中文摘要: |
| 从山东东营石油污染土壤中驯化筛选出一株正十六烷降解菌 TZSX2，经生理生化和 16S rDNA 基因测序，通过构建细菌系统发育树确定其为红球菌属（Rhodococcus）。通过不同环境因子对 TZSX2 的生长情况和其对正十六烷的降解率的影响研究，确定菌株 TZSX2 的最适生长和降解温度为 28~36 ℃，对正十六烷的降解率超过 30%；TZSX2 能够耐受较高浓度的正十六烷，在正十六烷浓度为 2 mL·L-1 时，降解率为 79%，正十六烷浓度为 20 mL· L-1 时，降解率仍可达到 12%；在碱性条件（pH=9）下对初始浓度为 10mL·L-1 的正十六烷的降解率高达 91%。综上，所筛选的 TZSX2 菌株可以耐碱性，适用于极端环境中石油污染的修复，对高浓度的正十六烷具有优异的降解效果。 |
| 英文摘要: |
| Samplings were performed in the petroleum contaminated soils of Dongying, Shandong Province of China. Degrading bacteria was isolated through enrichment in a Bushnel-Hass medium, with n-hexadecane as the sole source of carbon and energy. Then the isolated strains were identified by amplification of 16S rDNA gene and sequencing. The strain TZSX2 was selected as the powerful bacteria with stronger degradation ability, which was then identified as Rhodococcus hoagii genera based on the constructing results of the phylogenetic tree. The optimum temperature that allowed both high growth and efficient degradation ratio was in the scope of 28~36 ℃, and gas chromatography results showed that approximately more than 30% of n-hexadecane could be degraded in one week of incubation within the temperature range. Moreover, the strain TZSX2 was able to grow in high concentrations of n-hexadecane. The degradation rate reached 79% when the initial n-hexadecane concentration was 2 mL·L-1,while it still achieved 12% with n-hexadecane concentration of 20 mL·L-1. The optimal pH was 9 that allowed the highest growth and the greatest degradation rate of 91%. Above all, the screened strain TZSX2 showed high capabilities of alkali tolerance with excellent degradation efficiency for even high concentration of n-hexadecane, and thus it would be quite suitable for the remediation of petroleum contaminated soils especially in the extreme environment. |
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