文章摘要
小花南芥相关转运蛋白及有机酸对锌迁移的响应
Response of relative transporter and organic acid to Zn distribution in Arabis alpina L. var. parviflora Franch
投稿时间:2022-09-20  修订日期:2022-11-28
DOI:10.13254/j.jare.2022.0659
中文关键词: 小花南芥,Zn,果胶,转运蛋白,有机酸
英文关键词: Arabis alpina, Zn, pectin, transporter protein, organic acid
基金项目:国家自然科学基金项目(41867055);云南省农业联合专项(202101BD070001-047)
作者单位E-mail
廖雨梦 云南农业大学资源与环境学院, 昆明 650201  
邓随枫 云南农业大学资源与环境学院, 昆明 650201  
李祖然 云南农业大学园林园艺学院, 昆明 650201  
王磊 云南农业大学资源与环境学院, 昆明 650201  
王吉秀 云南农业大学资源与环境学院, 昆明 650201  
祖艳群 云南农业大学资源与环境学院, 昆明 650201 zuyanqun@qq.com 
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中文摘要:
      为探讨锌(Zn)在超富集植物中的迁移转运特征,采用小花南芥作为供试植物,通过盆栽试验,研究了外源添加不同浓度Zn(0、100、300 mg·kg-1)对小花南芥体内果胶含量、果胶甲酯酶(PME)活性、阳离子/H+反向运输体(CAX)活性、重金属ATP酶(HMA)活性、ATP结合盒转运蛋白(ABC)活性和有机酸(草酸、柠檬酸)含量的影响。结果表明:随Zn处理浓度的增加,叶部Zn含量呈上升趋势,根部Zn含量在100 mg·kg-1 Zn处理达到最大,且Zn含量在植株中的分布表现为根>叶。300 mg·kg-1 Zn处理下,共质体汁液中的Zn含量比质外体汁液中的Zn含量高85.80%,韧皮部汁液中的Zn含量比木质部汁液中的Zn含量高4.56倍。随Zn处理浓度的增加,根中果胶含量下降;与对照处理相比,2个Zn处理根和叶中PME活性增大。300 mg·kg-1 Zn处理下,叶中CAX和HMA活性达到最高,与对照相比分别增加了52.54%和38.73%。100 mg·kg-1 Zn处理下,根中草酸含量最低,叶中草酸含量最高。300 mg·kg-1 Zn处理下,柠檬酸含量在木质部汁液和叶中的含量最高,分别比对照增加161.10%和107.88%。小花南芥根部Zn含量与PME活性呈极显著正相关,与HMA、ABC活性和草酸含量呈显著负相关。小花南芥叶中Zn含量与CAX、HMA活性和木质部柠檬酸含量呈极显著正相关。研究表明,小花南芥通过降低果胶含量、增强膜转运蛋白活性以及柠檬酸含量来促进体内Zn的转运。
英文摘要:
      To investigate Zn translocation distribution of hyperaccumulator Arabis alpina L. var. parviflora Franch, a pot experiment was conducted to study the effects of different concentrations of Zn(0, 100 mg · kg-1, and 300 mg · kg-1)on the pectin content, pectin methylesterase(PME)activity, cation/ proton exchanger(CAX), heavy metal ATPase(HMA), and ATP-binding cassette protein(ABC) activities, and organic acid(oxalic and citric acid)contents of A. alpina. The results showed that the Zn contents in leaves tended to increase with the increase of Zn treatment concentrations. The Zn contents in roots reached the maximum at 100 mg·kg-1 Zn treatment, and the distribution of Zn content in plants was greater in roots than in leaves. With 300 mg·kg-1 Zn treatment, the Zn content in the symplast sap was 85.80% higher than that in the apoplast sap, and the Zn content in the phloem sap was 4.56 times higher than that in the xylem sap. The pectin content in roots decreased with the increase of Zn treatment concentrations, and PME activities increased in roots and leaves compared with the control treatment. The highest activities of CAX and HMA in the leaves were achieved with 300 mg · kg-1 Zn treatment, which increased by 52.54% and 38.73%, respectively, compared to the control. Oxalic acid content was the lowest in roots and highest in leaves with 100 mg·kg-1 Zn treatment. Under 300 mg·kg-1 Zn treatment, citric acid content was the highest in xylem sap and leaves, increasing by 161.10% and 107.88% compared to the control, respectively. The Zn contents in the roots of A. alpina showed a highly significant positive correlation with PME activity, and a significant negative correlation with HMA and ABC activities and oxalic acid content. The content of Zn in shoots of A. alpina showed a highly significant positive correlation with CAX and HMA activities and xylem citric acid content. In general, the results indicated that Zn in A. alpina transportation is relative to reducing pectin content and enhancing membrane transporter protein activities and citric acid contents.
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