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| 不同茶树品种吸收累积镉的差异研究 |
| Study on the difference between cadmium absorption and accumulation in different tea varieties |
| 投稿时间：2020-06-24 |
| DOI：10.13254/j.jare.2020.0336 |
| 中文关键词: 茶树品种，镉，吸收，累积，镉相关转运蛋白 |
| 英文关键词: tea varieties, cadmium, absorption, accumulation, Cd-related transport protein |
| 基金项目:现代农业产业技术体系建设专项资金（CARS-19）；中国农业科学院科技创新工程（CAAS-ASTIP-2016-TRICAAS） |
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| 中文摘要: |
| 为了解不同品种茶树对镉的响应差异，比较了9个当前主栽茶树品种对Cd吸收累积的差异特征。采用盆栽试验方法研究了不同品种茶树在对照、Cd处理（土壤Cd含量1 mg·kg-1）条件下，茶树地上部分干物质质量、Cd在茶树各部位的含量、富集转运系数以及茶树根部镉相关转运蛋白基因的表达。结果表明，Cd处理下不同茶树品种地上部分干物质质量出现不同程度增加。两种处理下，Cd在茶树中的分布趋势均表现为根 > 枝干 > 成熟叶 > 新梢，Cd处理下，这四个部位平均Cd含量分别为32.79、0.293、0.112、0.044 mg·kg-1。Cd处理下，土-根的富集系数和根-枝干、根-成熟叶、根-新梢的转移系数范围依次为7.17~30.12、0.017~0.078、0.002~0.007、0.000 3~0.006 1。CsZIP1、CsZIP2、CsHMA2、CsCAX2在紫鹃中表达量最高，CsHMA1在中茶108、乌牛早、紫鹃、浙农117表达差异不显著。综上，当茶园土壤0.3≤Cd≤1.5 mg·kg-1时，不同品种茶树的新梢Cd含量都远低于国家标准限值（Cd≤1 mg·kg-1），处于安全范围内。 |
| 英文摘要: |
| In order to understand the mechanisms of cadmium absorption and accumulation in different tea varieties, pot experiments were conducted to investigate dry weight in aboveground part of different tea plant varieties, Cd content, accumulation and translocation of Cd from soil to different parts of tea plant, and gene expression of Cd-related transport proteins in roots with and without Cd treatment. Results showed that dry weight of aboveground part of different tea plant varieties increased with Cd content. The Cd content in different tea varieties was in order of roots > branches > mature leaves > young leaves. The bioaccumulation factor of Cd in roots of plants treated with Cd ranged from 7.17 to 30.12. The biological transfer factor of Cd from roots to branches, mature leaves, and young leaves in Cd-treated plants ranged from 0.017~0.078, 0.002~0.007, and 0.000 3~0.006 1, respectively. Results showed that the expression of CsZIP1, CsZIP2, CsHMA2, and CsCAX2 was increased in Zijuan, whereas the difference in the expression of CsHMA1 among the four tea varieties (Zhongcha108, Wuniuzao, Zijuan, and Zhenong117) was not significant. In conclusion, when Cd content in soil is more than 0.3 mg·kg-1 and less than 1.5 mg·kg-1, we may consider young leaves as safe. |
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