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| 土壤中细菌HC5的分离鉴定及抑菌活性测定 |
| Isolation and identification of bacterium HC5 from soil and determination of antibiotic activity |
| 投稿时间：2018-01-10 |
| DOI：10.13254/j.jare.2018.0013 |
| 中文关键词: 土壤，细菌，HC5，假单胞菌，马铃薯晚疫疫霉菌，抑菌活性 |
| 英文关键词: soil, bacteria, HC5, Pseudomonas sp., Phytophthora infestans, antibiotic activity |
| 基金项目:甘肃省农业科学院农业科技创新专项（2015GAAS36，2014GAAS30） |
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| 中文摘要: |
| 为了筛选出对马铃薯晚疫疫霉菌（Phytophthora infestans）有较强拮抗作用的生防细菌，从马铃薯种植地的土壤中分离细菌，并通过平板对峙法，测定分离细菌对马铃薯晚疫疫霉菌的拮抗作用。经大量拮抗实验，筛选出一株细菌HC5，经形态特征观察、生理生化特性测定和16S rRNA基因序列分析，确定该菌株为假单胞菌（Pseudomonas sp.）。抑菌试验结果表明，HC5菌株对辣椒疫霉菌（Phytophthora capsici）、黄瓜尖孢镰刀菌（Fusarium oxysporum）、马铃薯晚疫疫霉菌、辣椒炭疽菌（Colletotrichum capsici）均有一定的抑制作用，尤其对马铃薯晚疫疫霉菌的抑菌效果显著，抑菌率达89%。采用PCR方法对菌株HC5进行多种抗生素合成基因的检测，扩增到786 bp的硝吡咯菌素片段和587 bp的氢氰酸片段，表明菌株HC5能够代谢产生这两种抗生素，单一或协同发挥拮抗作用。研究表明，菌株HC5是一株具有开发潜力的生防细菌。 |
| 英文摘要: |
| Lots of bacteria were isolated from the soil of the potato plantation, in order to screen out biocontrol bacteria with strong antagonistic efficacy on Phytophthora infestans by dual culture. We screened out a strain HC5 through lots of antagonistic tests. This strain was identified as Pseudomonas sp. by analysis of morphological, physiological and biochemical characteristics and 16S rRNA gene sequence. The results showed that the strain HC5 exhibited antibiotic activity of antagonists against Phytophthora capsici, Fusarium oxysporum, P. infestans and Colletotrichum capsici, especially it produced the outstanding antibiotic activity against P. infestans, with inhibition rate up to 89%. Detected multiple antibiotic synthesis gene in strain HC5 with PCR method, we amplified the 786 bp gene of PRN and 587 bp gene of HCN. This study showed that the strain HC5 could metabolize to produce the two antibiotics, playing the antagonistic role by itself only or coordination. Above of all, the strain HC5 was a biocontrol bacterium with potential development. |
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