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| Effects of turning frequency on antibiotic resistance genes and bacterial communities in dairy manure ectopic fermentation bed |
| Received:October 26, 2022 Revised:February 23, 2023 |
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| KeyWord:manure and sewage of dairy cattle;ectopic fermentation bed;antibiotic resistance genes;bacterial communities |
| Author Name | Affiliation | E-mail | | GAO Daoyu | Institute of Animal Husbandry and Veterinary Medicine, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China
College of Animal Science and Technology, Yangtze University, Jingzhou 434025, China | | | CUI Huawei | Institute of Animal Husbandry and Veterinary Medicine, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China
Animal Science and Technology College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China | | | ZHOU Yuan | Institute of Animal Husbandry and Veterinary Medicine, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China | | | GONG Ping | Institute of Animal Husbandry and Veterinary Medicine, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China | | | CHEN Jie | Institute of Animal Husbandry and Veterinary Medicine, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China | | | WAN Pingmin | Institute of Animal Husbandry and Veterinary Medicine, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China | | | SHAO Zhiyong | Institute of Animal Husbandry and Veterinary Medicine, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China | | | HUA Juan | Institute of Animal Husbandry and Veterinary Medicine, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China | | | LI Peng | College of Animal Science and Technology, Yangtze University, Jingzhou 434025, China | lipeng@yangtzeu.edu.cn | | JIN Erguang | Institute of Animal Husbandry and Veterinary Medicine, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China | 523229599@qq.com |
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| Abstract: |
| To investigate the effect of turning frequency on antibiotic resistance genes(ARGs), integrator genes(intI1), and bacterial communities during dynamic composting in ectopic fermentation beds, two conditions were established:turning over once per day(T1 group) and turning over once every two days(T2 group). The dynamic changes of target genes(tetG, tetW, sul1, sul2, blaTEM-1, ermQ, and intI1) and bacterial communities were studied using qPCR and 16S rRNA, and the relationship between ARGs, bacterial communities, and intI1 was analyzed. The results showed that the total relative abundance of target genes in T1 and T2 groups decreased by 82.33% and 80.46%, respectively, compared with 0 d after composting. The relative abundance of tetG, tetW, sul1, blaTEM-1, ermQ, and intI1 decreased by 16.70%, 87.88%, 54.60%, ＞99.99%, 97.80%, 59.33% in T1 and 61.33%, 99.46%, 50.91%, 99.29%, 82.23%, 99.92% in T2, respectively. Network analysis revealed that Trichococcus, Aquabacterium, and Clostridiaceae_Clostridium were common potential host bacteria for ARGs and intI1. Redundancy analysis showed that the bacterial communities accounted for 70.07% of the variation in ARGs, and intI1 explained 25.10%. The result showed that the succession of bacterial communities and the change in relative abundance of intI1 may be the key factors affecting the change in relative abundance of ARGs during the composting process of the ectopic fermentation bed, and the abundance of most ARGs could be reduced under both treatments. The removal efficiency of most ARGs was better in the condition of turning over once every two days; however, tetG, sul1 and sul2 could not be effectively removed. |
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