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| Combined extraction of bioavailability of arsenic and selenium in soils |
| Received:October 05, 2022 Revised:December 16, 2022 |
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| KeyWord:soil;arsenic;selenium;bioavailability;combined extraction |
| Author Name | Affiliation | E-mail | | WU Mei | Key Laboratory of GIS Application Research, Chongqing Normal University, Chongqing 401331, China
School of Geography and Tourism, Chongqing Normal University, Chongqing 401331, China | | | ZHAO Jiayu | Key Laboratory of GIS Application Research, Chongqing Normal University, Chongqing 401331, China
School of Geography and Tourism, Chongqing Normal University, Chongqing 401331, China | | | LIU Yi | Key Laboratory of GIS Application Research, Chongqing Normal University, Chongqing 401331, China
School of Geography and Tourism, Chongqing Normal University, Chongqing 401331, China | | | TIAN Xinglei | Shandong Institute of Geological Sciences, Jinan 250013, China | | | NI Runxiang | Rural Energy and Environment Agency, Ministry of Agriculture and Rural Affairs, Beijing 100125, China | | | LIU Yonglin | Key Laboratory of GIS Application Research, Chongqing Normal University, Chongqing 401331, China
School of Geography and Tourism, Chongqing Normal University, Chongqing 401331, China | liu3986130@163.com |
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| Abstract: |
| In this study, to improve experimental efficiency by reducing the number of experimental steps, a rapid, accurate, and effective method for extracting bioavailable As and Se in soils was designed, produced, and evaluated. The experimental effects of four extractants （0.5 mol·L-1 NaHCO3, 0.1 mol·L-1 HCl, 0.5 mol·L-1 KH2PO4 and 0.1 mol·L-1 KH2PO4-K2HPO4）in extracting bioavailable As and Se in soils were compared. Following the mixture and extraction process, the resultant solutions were added to a mixed acid solution of HNO3 and HClO4 and further digested. Subsequently, a hydride generation atomic fluorescence spectrometer（HG-AFS）was used to determine the content of As and Se. Additionally, soil reference materials were used as controls for determining total As and total Se in soils. The experimental quality of corn root and corn seed was controlled using the corn reference material. The relative standard deviation was within 10%, and the detection limits for Se and As were 0.01 μg·L-1and 0.05 μg·L-1, respectively. We found that the contents of soil bioavailable As and Se extracted using 0.1 mol·L-1 KH2PO4-K2HPO4 and 0.5 mol·L-1 NaHCO3 buffer solutions were positively correlated with the total As（r=0.788, P<0.01; r=0.794, P<0.01）and total Se（r=0.707, P<0.05; r=0.648, P<0.05）in soils, whereas the other two extracts were ineffective. The plant index method showed that the content of soil bioavailable As and Se extracted using 0.1 mol·L-1 KH2PO4-K2HPO4 buffer solution had a significant positive relationship only with that of corn root（r=0.848 for As, P<0.01 and r=0.822 for Se, P<0.01）. In conclusion, 0.1 mol·L-1 KH2PO4-K2HPO4 buffer solution could be used as an extractant to rapidly, accurately, and effectively extract bioavailable As and Se in soils. |
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