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| Response characteristics of bacterial communities to chromium stress in the soil of millet fields |
| Received:March 16, 2023 Revised:May 11, 2023 |
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| KeyWord:millet field;chromium stress;bacterial community;community composition;community assembly process |
| Author Name | Affiliation | E-mail | | JING Xiuqing | College of Biological Sciences and Technology, Taiyuan Normal University, Jinzhong 030619, China
College of Life Science, Shanxi University, Taiyuan 030006, China | | | ZHAO Pengyu | College of Biological Sciences and Technology, Taiyuan Normal University, Jinzhong 030619, China | zpy060033@126.com | | BAI Xue | College of Biological Sciences and Technology, Taiyuan Normal University, Jinzhong 030619, China | | | DENG Ning | College of Biological Sciences and Technology, Taiyuan Normal University, Jinzhong 030619, China | | | ZHAO Xiaodong | College of Biological Sciences and Technology, Taiyuan Normal University, Jinzhong 030619, China | | | WU Lihua | College of Biological Sciences and Technology, Taiyuan Normal University, Jinzhong 030619, China | | | YI Huilan | College of Life Science, Shanxi University, Taiyuan 030006, China | |
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| Abstract: |
| To investigate the response mechanism of bacterial communities in farmland soil to chromium（Cr）stress, soil planted with “Jingu 21”millet（Setaria italica）was used as the experimental material in this study. Samples were acquired before Cr stress（CK group） and after 6 h（Cr_6h）and 6 d（Cr_6d）of 1 mmol·L-1 Cr6+ stress. The composition, diversity, assembly process, and KEGG metabolic pathway activity of bacterial community in the soil under Cr stress were analyzed using Illumina MiSeq high-throughput sequencing. The results revealed that there were significant differences in the proportion of dominant bacteria at phylum and genus levels in the bacterial communities under different durations of Cr stress. The α diversity varied in stages, for example the Shannon index decreased at Cr_6h and increased at Cr_6d（6.07 at CK, 5.92 at Cr_6h, and 6.04 at Cr_6d）, whereas the Simpson index exhibited the opposite trend（0.006 8 at CK, 0.007 8 at Cr_6h, and 0.006 8 at Cr_6d）. After Cr stress, the beta NTI index continued to decrease（-2.68 at CK, -2.11 at Cr_6h, and -1.91 at Cr_6d）. The bacterial community assembly processes in CK and Cr_6h stage were dominated by deterministic assembly process （|beta NTI|>2）, whereas in Cr_6d stage, they were mainly dominated by stochastic assembly process（|beta NTI|<2）. The number of edges and average degree of bacterial communities decreased significantly, whereas the average path length continued to increase significantly. The symbiosis of the bacterial community in the same module was mainly positively correlated. The above results demonstrated that Cr stress significantly affects the bacterial community in the soil of millet fields. With the duration of Cr stress, the bacterial community assembly processes gradually changed from being dominated by deterministic assembly process to being dominated by stochastic assembly process. The symbiotic network of bacteria became smaller, and the response rate became slower, which was dominated by a symbiotic relationship. The scale of the symbiotic network of bacterial communities decreased, and the response speed between bacterial communities slowed down; the symbiotic relationship was the dominant factor. |
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