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Effects of pretreatment by freeze-vacuum drying on the biochemical methane potential of waste cured tobacco leaves
Received:July 30, 2021  
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KeyWord:anaerobic digestion;waste cured tobacco leave;freeze-vacuum drying;biochemical methane potential
Author NameAffiliationE-mail
XU Jianxue College of Agronomy, Hunan Agricultural University, Changsha 410128, China
Hunan BISEN Environmental & Energy Co., Ltd., Changsha 410100, China 
 
XUE Hanguang Hunan BISEN Environmental & Energy Co., Ltd., Changsha 410100, China  
HUANG Hongyi College of Agronomy, Hunan Agricultural University, Changsha 410128, China  
HE Yuewei Hunan BISEN Environmental & Energy Co., Ltd., Changsha 410100, China  
HAN Rui Qinghai Key Laboratory of Vegetable Genetics and Physiology, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China  
PENG Xing Hunan United Kitchen Waste Treatment Co., Ltd., Changsha 410022, China  
LI Juan College of Agronomy, Hunan Agricultural University, Changsha 410128, China
Hunan BISEN Environmental & Energy Co., Ltd., Changsha 410100, China 
adalee619@163.com 
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Abstract:
      This study aimed to explore the effect of pretreatment by freeze-vacuum drying on the biochemical methane potential(BMP)of waste cured tobacco leaves, in order to improve their utilization and promote the development of a greener and cleaner production system that would help tobacco companies around the world fulfil their pledges on carbon neutrality. Waste cured tobacco leaves were pretreated by freeze-vacuum drying for 0, 1, 3, 5 h, and 10 h. The surface microstructures, lignocellulosic structures, total sugar contents, and nicotine contents of the samples were compared, and their BMP were calculated. The BMP of waste cured tobacco leaves were 200.74, 208.64, 220.98, 224.28, 211.11 mL·g-1, and 209.30 mL·g-1(calculated by CH4 production per gram of volatile solid), respectively. Compared with that of untreated leaves, the BMP of waste cured tobacco leaves pretreated for 0, 1, 3, 5 h, and 10 h increased by 3.94%, 10.08%, 11.73%, 5.16%, and 4.26%, respectively. After freeze-vacuum drying, the surface of the leaves became wrinkled and curled, and presented a loose and porous physical structure, which could effectively increase the area of contact with microorganisms. However, long-term freezevacuum drying not only increased the energy consumption to a large extent, but also caused the loss of organic components of the material. Finally, the recommended pretreatment time was calculated based on the balance between the energy derived from methane production and that required for freeze-vacuum drying, which is essential for assessing the sustainability of this process.