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| Pig manure amendment improves the quality of products during composting of solanaceous vegetable waste： Insights into the microbial mechanism |
| Received:July 22, 2021 |
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| KeyWord:solanaceous vegetable waste;aerobic composting;microbial community;metabolic function |
| Author Name | Affiliation | E-mail | | LU Xiaolin | Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | | | YANG Yuxin | Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China | | | HONG Chunlai | Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | | | ZHU Weijing | Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | | | ZHU Fengxiang | Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | | | YAO Yanlai | Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | | | HONG Leidong | Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | | | WANG Weiping | Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | wangweiping119@126.com |
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| Abstract: |
| The structure and metabolic functions of the microbial community during solanaceous vegetable waste composting with the addition of pig manure were studied. This study aimed to provide new insights into the mechanism of nutritional quality improvement through the use of animal manure as amendment during composting. Solanaceous vegetable waste was mixed well with the tested straw compost. An aerobic composting experiment was conducted indoors for 45 days. Test composting piles were treated with pig manure（EPM）, while untreated piles served as control（E）. Compost samples were collected, and their physicochemical parameters, germination indices, and enzymatic activities were monitored at days 0, 2, 9, 16, 23, 31, 38, and 45 of the composting process. Moreover, fresh compost samples were collected on the 2nd, 23rd, and 38th day after the start of composting, and the structure and metabolic functions of the microbial community were analyzed by 16S rRNA and 18S rRNA high-throughput sequencing. The addition of pig manure resulted in a relatively higher abundance of Proteobacteria and Chloroflexi in the early stage, an increased pile temperature, a longer duration of the thermophilic stage, and a higher germination index（82.11%, P<0.05）. Compared to the E treatment, E-PM treatment significantly enhanced total phosphorus and total potassium contents in the piles（P<0.05）. The analysis of enzymatic activity demonstrated that E-PM treatment significantly promoted（P<0.05）the activity of proteases, polyphenol oxidases, and cellulases, as well as the degradation of organic matter, during the thermophilic stage. Moreover, high-throughput sequencing and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States revealed that, compared to that of E-treated piles, the abundance of bacterial genes related to metabolism and cellular processes of E-PM-treated piles increased by 5.68% and 10.26%, respectively, during the thermophilic stage. The abundance of genes related to carbohydrate metabolism, amino acid metabolism, energy metabolism, and enzymatic activity related to organic matter degradation also increased greatly with pig manure addition. Furthermore, correlation analysis showed that the higher relative abundance of Proteobacteria and Chloroflexi influenced the metabolic characteristics of the thermophilic compost under E-PM treatment, which accelerated the maturation of the compost product. Altogether, our results indicate that the addition of pig manure led to increased metabolic functions of the bacterial community in the thermophilic stage, significantly accelerated the rise of pile temperature, and improved the quality of end products |
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