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| Effects of different fertilization modes on the pollution of antibiotic resistance genes in paddy soils of Erhai Lake basin |
| Received:September 08, 2021 |
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| KeyWord:manure application;fertilization mode;antibiotic resistance gene;pollution spectrum;distribution patterns |
| Author Name | Affiliation | E-mail | | HAN Bingjun | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | SHEN Shizhou | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
Dali Observation and Experimental Station of National Agricultural Environment, Dali 671004, China | | | YANG Fengxia | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | yangfengxiacomeon@163.com | | GAO Wenxuan | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | DING Yongzhen | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | ZHANG Keqiang | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | keqiangzhang68@163.com |
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| Abstract: |
| To explore the effects of different fertilization modes on antibiotic resistance genes(ARGs)and mobile genetic elements(MGEs) in paddy soils of Erhai Lake basin, 144 ARGs and 10 MGEs in paddy soil under different fertilization modes were detected using high-throughput quantitative PCR and real-time quantitative PCR to comprehensively analyze the pollution status and spatial distribution of ARGs in paddy fields. The results showed that the distribution patterns of ARGs were different in different fertilization treatments; the absolute abundance of ARGs in the soil was in the order of manure application > slow and controlled release fertilizer application > chemical fertilizer treatment > no fertilization. The occurrence and diversity of ARGs and the types of MGEs in soil with manure application were significantly higher than those in other treatments, whereas those in the non-fertilization treatment were significantly lower than those in other treatments. The relative abundance of different ARGs showed a vertical migration and enrichment trend in the paddy soil column, but their enrichment degree was different in different soil layers. The dominant ARGs in each paddy soil layer under different fertilization modes were multidrug(average proportion of 60.3%), aminoglycoside(average proportion of 10.5%), and tetracycline(average proportion of 7.5%). Their levels were significantly correlated with the abundance of MGEs(P<0.05), thereby indicating that MGEs promoted the spread of ARGs and aggravated the accumulation and enrichment of ARGs in the soil. The distribution of ARGs and MGEs in paddy soil was significantly affected by different fertilization modes, and the enrichment degree of ARGs in different soil layers was different. |
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