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| Mechanisms of bacterial community response to hypothermia-enhanced compost start-up process |
| Received:September 26, 2021 |
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| KeyWord:psychrophile;composting start-up;microbial community;winter composting |
| Author Name | Affiliation | E-mail | | XIE Xinyu | College of Life Science, Northeast Agricultural University, Harbin 150030, China | | | ZHAO Yue | College of Life Science, Northeast Agricultural University, Harbin 150030, China | | | ZHANG Shubo | College of Life Science, Northeast Agricultural University, Harbin 150030, China | | | YANG Hongyu | College of Life Science, Northeast Agricultural University, Harbin 150030, China | | | WEI Zimin | College of Life Science, Northeast Agricultural University, Harbin 150030, China | weizimin@neau.edu.cn | | ZHANG Xu | College of Life Science, Northeast Agricultural University, Harbin 150030, China | | | ZHAO Li | College of Life Science, Northeast Agricultural University, Harbin 150030, China | |
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| Abstract: |
| The response mechanisms of psychrophiles to enhance the bacterial community in northern winter organic waste composting and ensure rapid start-up of organic waste composting in cold environments was explored. This study conducted a rapid start-up experiment of livestock manure compost enhanced by psychrophiles. Moreover, combined with 16S rRNA amplicon sequencing technology and bioinformatics analysis methods, changes in piles temperature, microhabitat factors, and microbial characteristics were determined to explore the role of bio-enhanced psychrophiles in the start-up process of livestock manure compost and to investigate the microbial response mechanism of the composting process. The results showed that the temperature of the piles inoculated with the inoculation treatment(CT)rose rapidly to 20.0℃ at 18 h and quickly crossed the start-up period when the temperature of the uninoculated control (CK)group was only 15.8℃. The temperature in the CT group exceeded 50.0℃ at 36 h. The treatment group that was not inoculated with psychrophiles could not complete the rapid start-up of the composting. Moreover, the rapid decomposition of organic fractions in the CT group provided sufficient thermal energy for the increase in composting temperature. It was clear from the changes in community abundance that psychrophiles were biomarkers that promote changes in bacterial communities and that inoculation with psychrophiles activated the evolution of bacterial community structure during compost start-up. In addition, the results of the variance partitioning analysis showed that the interaction of psychrophiles, physicochemical indicators, and bacterial communities were the dominant factors in the temperature variation of the compost and that the temperature variation of the compost start-up process could be regulated by increasing the abundance of psychrophiles. |
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