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| Laccase mediated jute enhancement for the removal of methylene blue from water |
| Received:December 13, 2020 |
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| KeyWord:laccase-jute composite;adsorption;enzymolysis;methylene blue;regeneration |
| Author Name | Affiliation | E-mail | | SUN Xiuxian | College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China | | | MENG Zhaofu | College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China
Key Laboratory of Plant Nutrition and Agro-Environment in Northwest China, Ministry of Agriculture and Rural Affairs, Yangling 712100, China | zfmeng1996@263.net | | CAO Xuewen | College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China | | | WANG Teng | College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China | | | HU Xiaolong | College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China | | | WANG Xinxin | College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China | | | LIU Xin | College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China | |
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| Abstract: |
| In this study, laccase was loaded in a segment jute（SJ）matrix, and a series of laccase-jute composite materials（0.1%L-SJ, 0.5%L-SJ, 1%L-SJ, 5%L-SJ）were produced. The effects of laccase dosage, temperature, pH, ionic strength, and regeneration on the efficiency to remove methylene blue（MB）were studied using the batch method. Furthermore, Fourier Transform Infrared Spectroscopy, Scanning Electronic Microscopy, and the surface charge were assessed to characterize and explore the mechanism of MB removal. The results indicated that the laccase-loaded jute exhibited enhanced MB removal ability compared with that of raw jute. The order of MB removal rate was 1%L-SJ > 5%L-SJ > 0.5%L-SJ > 0.1%L-SJ>SJ, exhibiting a trend of first increasing and then decreasing. Additionally, the MB removal by the five materials conformed to the Sips adsorption isotherm model, where 1%L-SJ exhibited the highest removal rate of 95.12% and the fitted adsorption capacity（qS）was 1 097.11 mmol·kg-1. Furthermore, the removal process was spontaneous, and the increase in temperature and ionic strength and a decrease in pH were not conducive to MB removal. Finally, 1%L-SJ maintained a removal rate of 76.66% after being regenerated four times. The mechanism of MB removal by the laccase-jute composite was found to the synergistic action of jute adsorption and laccase enzymatic hydrolysis. Jute adsorption contributed to 91.12% of MB removal, while enzymatic hydrolysis of laccase contributed to 8.88% of MB remval. The adsorption mechanisms of jute mainly involved electrostatic interaction and hydrogen bonding, whereas laccase improved the MB removal by enzymatic hydrolysis and altering the jute structure. |
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