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| Isolation and characterization of high-efficiency glyphosate-degrading bacteria |
| Received:December 06, 2020 |
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| KeyWord:glyphosate;bioremediation;Ensifer sp.;degrading bacteria;Haldane's model |
| Author Name | Affiliation | E-mail | | WANG Tian-kuo | Key Lab of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun 130021, China | | | WEN Yu-juan | Key Lab of Eco-restoration of Regional Environment Pollution, Shenyang University, Shenyang 110044, China | | | YANG Yue-suo | Key Lab of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun 130021, China
Key Lab of Eco-restoration of Regional Environment Pollution, Shenyang University, Shenyang 110044, China | yangyuesuo@jlu.edu.cn | | LU Ying | Key Lab of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun 130021, China | | | ZHANG Xi | College of Water Conservancy and Civil Engineering, Northeast Agricultural University, Harbin 150030, China | | | CAO Nan | Chengdu Center of Hydrogeology and Engineering Geology, SBGEEMR, Chengdu 610081, China | | | SUN Dong | Chengdu Center of Hydrogeology and Engineering Geology, SBGEEMR, Chengdu 610081, China | |
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| Abstract: |
| Pollution from pesticides is a primary eco-health concern, in which glyphosate eco-treatment is a particularly controversial topic. In this study, an efficient glyphosate-degradation bacteria Ensifer sp. BRY was isolated and screened from farmland soil in Shenyang, China, where various pesticides have been applied for extended periods. Strains were identified by 16s rDNA sequence homology analysis. The optimal growth and glyphosate removal characteristics of strain BRY were explored by an environmental singlefactor experiment. Kinetic curve fitting was performed on the removal processes of different initial glyphosate mass concentrations using the Haldane equation. Results show that the strain BRY identified as Ensifer sp. BRY can grow in an inorganic salt medium with glyphosate (maximum concentration of 400 mg·L-1) as the sole carbon and energy source. The glyphosate degradation rate(300 mg·L-1) can reach 69.60% within 50 h. The glyphosate degradation rate(100 mg·L-1) of strain BRY reached 91.93% within 50 h at 30℃, pH 6.0, and a 10% initial inoculation. The glyphosate degradation rate of strain BRY increased when the initial inoculum was adjusted to 20% under the same conditions and did not change significantly when the initial inoculum was increased to 30%, indicating that the optimal initial inoculation amount for BRY to degrade glyphosate was 20%. When other carbon sources(glucose, sucrose) were added to the culture system, the glyphosate degradation rate decreased. The glyphosate degradation process of strain BRY agrees with the Haldane equation. According to Haldane's model, the kinetic parameters for the glyphosate-grown strain BRY were μmax=1.68 h-1, Ks=167.80 mg·L-1, Ksi=50.55 mg·L-1, and Ksi/Ks=0.30. Based on these results, the strain BRY shows particular potential in the bioremediation of a glyphosate-contaminated environment. |
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