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| Effects of applying organic fertilizer from chicken excrement on the microbial community structural diversity in soils planted with Chinese cabbage（Brassica chinensis） |
| Received:January 08, 2020 |
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| KeyWord:organic fertilizer;soil;fertility;phospholipid fatty acid(PLFA);soil microbes;community structure |
| Author Name | Affiliation | E-mail | | LI Ke | Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | SUN Tong | Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | | | SUN Tao | Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
College of Grassland and Environment Sciences, Xinjiang Agricultural University, Urumqi 830052, China | | | XU Ying-ming | Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | SUN Yue-bing | Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | sunyuebing@aepi.org.cn |
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| Abstract: |
| To better understand the influence of different doses of organic fertilizer from chicken excrement on the soil microbial community diversity in soils planted with Chinese cabbage(Brassica chinensis), five levels (CK:0, T0.5:7.5 t·hm-2, T1:15 t·hm-2, T2:30 t·hm-2, and T4:60 t·hm-2)of organic fertilizer from chicken excrement were applied to the experimental plots. Field trials included three crops of Chinese cabbage. After the Chinese cabbages were harvested, soil samples were collected for analyzing the basic chemical properties and the microbial phospholipid fatty acid(Phospholipid fatty acid, PLFA) composition to investigate the changes in the microbial population and community structure and diversity, and the correlation between them when different levels of fertilizers were applied. Results show that the application of organic fertilizer from chicken excrement increased the soil fertility. The soil organic matter (SOM), total nitrogen(TN), total phosphorus(TP), total potassium(TK), nitrate nitrogen(nitrate-N), and available phosphorus(AP) increased respectively by 34.1%, 48.2%, 47.9%, 35.5%, 3.9%, and 14.7% in T4 over that in CK. The total PLFA content, that of each microbial group, and the fungus-to-bacteria PLFA ratios in middle and high fertilizer treatments were higher than that in the treatment without fertilizer. Among these treatments, the total PLFA content, the bacterial, and the fungal PLFA in T4 increased by 53.4%, 52.1%, and 108.3%, respectively in contrast to the control. The Shannon, Simpson, and Pielou indexes in T2 were the highest among the treatments. Microbial community structural diversity was similar among treatments with no fertilizer, extremely low, and low fertilizer applications(≤ 15 t·hm-2), and differed significantly from treatments with moderate and high fertilizer applications(≥ 30 t·hm-2). The results of redundant analysis(RDA) showed that the basic chemical characteristics of soil explained 87.2% of the changes in the community structural diversity. The main driving factors were nitrate-N, TP, and pH. Microbial PLFA were positively correlated with the AP, ammonium nitrogen(ammonium-N), nitrate-N, TN, and TP, while they were negatively correlated with soil CEC and pH. These results indicate that the fertility and microbial community diversity in soil can be improved by the application of organic fertilizer from chicken excrement. However, changes in soil microbial community structure and diversity varied according to different doses of organic fertilizer from chicken excrement. Fertilization rates of 30 t·hm-2 and 60 t·hm-2 were more effective for improving soil fertility and microbial amounts when compared to other treatment levels. Therefore, organic fertilizer derived from chicken excrement can be used to improve the soil fertility and biological quality by appropriately increasing the rate of application in a short time. |
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