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| Removal of ethinylestradiol in aqueous solution by immobilized Pseudomonas citronellolis SJTE-3 |
| Received:March 13, 2020 |
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| KeyWord:Pseudomonas citronellolis SJTE-3;17α-ethinylestradiol;immobilized bacterial agent;calcium alginate;microbial degradation |
| Author Name | Affiliation | E-mail | | WANG Yan-qiu | School of Life Sciences and Biotechnology, State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China | | | PENG Wan-li | School of Life Sciences and Biotechnology, State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China | | | LIANG Ru-bing | School of Life Sciences and Biotechnology, State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China | icelike@sjtu.edu.cn |
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| Abstract: |
| Ethinylestradiol(17α-ethynylestradiol, EE2)is an estrogenic drug and hormone additive that is commonly used in aquaculture and causes serious environmental estrogen pollution. The preparation and application of stable immobilized bacterial agents can achieve the effective removal of environmental EE2 pollution. In this study, the calcium alginate method was used to fix estrogen-degrading Pseudomonas citronellolis SJTE-3 in order to create immobilized bacterial agents with more stable degradation performance. The results of the orthogonal tests demonstrated that the best immobilization conditions were 3% sodium alginate solution, 4% CaCl2 solution, and 2 mL of bacterial solution with an OD600 of 10.0. The degradation efficiency of the optimized immobilized bacterial agent for an EE2 concentration of 10 μg·mL-1 in the Tris-HCl medium reached 78.8%, which was approximately 23.0% higher than that of the free bacterial agent. Meanwhile, the removal rate of EE2(5~50 μg·mL-1)in the simulated sewage by the immobilized bacterial agent was approximately 78%, which was significantly higher than that of the free bacterial agent. In addition, the immobilized bacterial agent could be used repeatedly with a long and stable degradation time. After the 10th use, the EE2 removal rate was still 53%. The immobilized Pseudomonas citronellolis SJTE-3 bacterial agent has a stable and efficient degradation capability, high environmental adaptability, and application potential for actual remediation. |
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