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| Effects of Tween 80 on colonization and performance of pyrene-degrading strain Pyr9 in Trifolium repens L. |
| Received:March 14, 2020 |
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| KeyWord:polycyclic aromatic hydrocarbon;Tween 80;pyrene-degrading bacterium;colonization;plant uptake |
| Author Name | Affiliation | E-mail | | CHEN Zhi-gao | College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China | | | GU Yu-jun | College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China | | | ZHANG Hai-li | College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China | | | LIU Juan | College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China | liujuan@njau.edu.cn | | GAO Yan-zheng | College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China | |
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| Abstract: |
| To investigate the colonization and performance of PAH-degrading bacterium in plants under nonionic surfactants, a series of greenhouse container experiments were performed with Tween 80, pyrene-degrading bacterium Pyr9, and white clover(Trifolium repens L.) as test subjects. The results showed that the cell counts of Pyr9 colonized in white clover increased initially and later decreased with the increase in Tween 80 content. Maximum count was observed when the content of Tween 80 was 100 mg·kg-1. A high content of Tween 80 (≥ 500 mg·kg-1)significantly inhibited the growth of white clover, but the inhibition was weakened by Pyr9 colonization. The addition of Tween 80 enhanced the pyrene uptake of white clover from soil, while the inoculation of Pyr9 significantly reduced the pyrene content in white clover. Furthermore, Tween 80 enhanced the removal efficiency of pyrene from soil under the combination of white clover and Pyr9. Proper dosage of Tween 80 promoted the colonization and performance of pyrene-degrading strain Pyr9 in white clover and enhanced the removal efficiency of pyrene in soil and plants. The results not only provide a theoretical basis for the safe production of agricultural products in PAH-contaminated areas, but also provide technical support for the "production while remediation" of PAH-contaminated soil. |
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