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| Effects of cadmium stress on mycelium physiological indexes and cadmium absorption in Agaricus brasiliensis |
| Received:April 24, 2020 |
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| KeyWord:cadmium stress;Agaricus brasiliensis;hyphae;physiological biochemistry;cadmium absorption |
| Author Name | Affiliation | E-mail | | LIU Peng-hu | National Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Fujian Key Laboratory of Agroecological Processes in Red Soil Hilly Region, Fuzhou 350003, China | | | CHEN Hua | Agricultural Ecology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
Fujian Key Laboratory of Agroecological Processes in Red Soil Hilly Region, Fuzhou 350003, China | | | LI Bo | Fujian Key Laboratory of Agroecological Processes in Red Soil Hilly Region, Fuzhou 350003, China | | | WANG Yi-xiang | Agricultural Ecology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
Fujian Key Laboratory of Agroecological Processes in Red Soil Hilly Region, Fuzhou 350003, China | sd_wolong@163.com | | WENG Bo-qi | Agricultural Ecology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
Fujian Key Laboratory of Agroecological Processes in Red Soil Hilly Region, Fuzhou 350003, China | |
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| Abstract: |
| To compare the effects of cadmium(Cd)stress on mycelia growth and Cd absorption of Agaricus brasiliensis strains J77 and J1, Cd was added to A. brasiliensis culture medium at concentrations ranging from 0 to 75 mg·L-1, and the physiological indexes and Cd content of the mycelia were measured. The results showed that the effects of Cd stress on the J77 and J1 mycelia antioxidant systems were different. As the Cd concentration(i.e., stress)increased, the activities of superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), ascorbate peroxidase(APX), and malondialdehyde(MDA)in the mycelia increased then decreased in both strains. At Cd concentrations greater than 5 mg·L-1, the SOD and POD activities of both strains decreased. POD activity of J77 and J1 mycelia decreased by 92.7% and 95.3%, respectively. When the Cd concentration was between 5 and 50 mg·L-1, CAT activity in J77 and J1 decreased by 40.9% and 60.4%, respectively, indicating greater sensitivity to Cd during J1 growth. When the Cd concentration was between 0 and 2 mg·L-1, APX activity increased in both strains as the concentration increased; J77 was 13 times higher, and J1 was 7.9 times higher. The J77 APX activity increase was nearly 5 times greater than J1. Under the same treatment conditions(0 to 75 mg·L-1 Cd), the MDA content in the mycelia was higher in J1 in than J77. In addition, a rapid increase of MDA and severe oxidation were observed in the J1 strain, while opposite patterns were observed in J77. The mycelia MDA content peaked at 15 mg·L-1 Cd in J77(60% higher than that of the control)and at 10 mg·L-1 Cd in J1(1.6 times higher than that of the control). These differences indicated that the cell membrane peroxidation of J1 mycelia was more severe. The Cd concentration in the mycelia increased with increasing environmental Cd(0 to 75 mg·L-1)in both strains. When the environmental Cd concentrations were between 2.5 and 50 mg·L-1, the Cd in J1 and J77 mycelia increased by 17.2 and 19.1 times, respectively. The Cd growth rate in the J77 mycelia was higher than J1, but the overall Cd content of the J1 mycelia was nearly 2.3 times higher. These results show that J77 has a higher tolerance to Cd stress and greater resistance to Cd absorption than J1. As the Cd content of the J77 and J1 strains increased, the SOD, POD, CAT, and APX enzyme activities, and MDA content increased then decreased. The Cd content in the J77 mycelia was lower than J1, indicating that J77 has low cadmium absorption potential. |
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