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| The effect of humic acid on arsenate toxicity of lettuce |
| Received:November 26, 2019 |
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| KeyWord:arsenate;humic acid;lettuce;growth performance;safety quality |
| Author Name | Affiliation | E-mail | | WANG Qi | Key Laboratory for Environmental Factors Control of Agro-product Quality Safety(Ministry of Agriculture and Rural Affairs), Tianjin Key Laboratory of Agro-environment and Safe-product, Institute of Agro-environmental Protection, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | WEN Jing-yu | Key Laboratory for Environmental Factors Control of Agro-product Quality Safety(Ministry of Agriculture and Rural Affairs), Tianjin Key Laboratory of Agro-environment and Safe-product, Institute of Agro-environmental Protection, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | ZHAO Yu-jie | Key Laboratory for Environmental Factors Control of Agro-product Quality Safety(Ministry of Agriculture and Rural Affairs), Tianjin Key Laboratory of Agro-environment and Safe-product, Institute of Agro-environmental Protection, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | YANG Ye | Key Laboratory for Environmental Factors Control of Agro-product Quality Safety(Ministry of Agriculture and Rural Affairs), Tianjin Key Laboratory of Agro-environment and Safe-product, Institute of Agro-environmental Protection, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | ZHU Jia-chao | Key Laboratory for Environmental Factors Control of Agro-product Quality Safety(Ministry of Agriculture and Rural Affairs), Tianjin Key Laboratory of Agro-environment and Safe-product, Institute of Agro-environmental Protection, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | MU Li | Key Laboratory for Environmental Factors Control of Agro-product Quality Safety(Ministry of Agriculture and Rural Affairs), Tianjin Key Laboratory of Agro-environment and Safe-product, Institute of Agro-environmental Protection, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | muli@caas.cn | | LIU Xiao-wei | Key Laboratory for Environmental Factors Control of Agro-product Quality Safety(Ministry of Agriculture and Rural Affairs), Tianjin Key Laboratory of Agro-environment and Safe-product, Institute of Agro-environmental Protection, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | xwliu2006@163.com | | KANG Wei-lu | Key Laboratory of Pollution Processes and Environmental Criteria(Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin, 300071, China | |
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| Abstract: |
| To explore the accumulation of arsenic in lettuce under different arsenate treatment conditions and the regulation of humic acid on arsenate toxicity of lettuce, the effect of the metabolism of lettuce on the pH value of culture medium and change of pH value under the synergistic action of humic acid and arsenate were analyzed. The accumulation of arsenate in lettuce was dynamically monitored by highperformance liquid chromatography in addition to inductively coupled plasma mass spectrometry. Furthermore, a chlorophyll analyzer, amino acid analyzer, and least partial square analysis were used to explore the growth performance and related quality indicators of lettuce and further study the regulation of humic acid on these indicators. The results showed that the pH value of the medium increased significantly in the non-arsenate treatment group and decreased with the increase of arsenate concentration. When the concentration of arsenate was 10 mg·L-1, the decrease in pH value was the most significant, with a value of 3.87. The addition of humic acid had a different mitigation effect degree on arsenate lettuce acid production. At the same time, the study found that the content of inorganic arsenic in lettuce tissues showed a different increasing trend with the increase of arsenate concentration in different growth stages. In the CK and CKHA groups, the arsenic species in the lettuce roots, stems, and leaves were mainly pentavalent arsenic. With the increase of humic acid concentration, the inorganic arsenic content decreased in the different growth stages of lettuce. However, in different arsenate treatment groups, the main form of arsenite in the lettuce plants was As(Ⅲ). When the concentration of arsenate was 0.5 mg·L-1, the biomass, plant height, root length, and relative content of chlorophyll were higher than the CK group, indicating that low concentrations of arsenate could promote the growth of lettuce. High concentrations of arsenate inhibited the growth of lettuce in varying degrees, while the addition of an appropriate concentration of humic acid promoted the growth of lettuce in varying degrees and alleviated the toxicity of high concentration arsenate in lettuce. In the CK, V-0.5 and V-1 treatment groups, the content of vitamin C and soluble sugar in the V-0.5 treatment group was the highest and the content of nitrate was the lowest, indicating that a low concentration of arsenate improved the quality of lettuce. In the arsenate treatment group, when the concentration of humic acid was 5 mg·L-1, the content of vitamin C and total phenols was the highest and the nitrate content was significantly lower than the V-10 treatment group, which could improve the quality of lettuce. Arsenate could decrease the pH of lettuce growth environment and humic acid could decrease the stress of acid production. When the concentration of arsenate was lower than 3.5 mg·L-1, the content of arsenic in lettuce leaves was not beyond the national limit standard at all growth stages and humic acid had a time-effect on the regulation of inorganic arsenic content in lettuce leaves. Humic acid could up-regulate the expression of glutamate and arginine related to stress resistance. |
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