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| Occurrence characteristics and risk assessment of resistance genes in livestock waste from family farms in Tianjin City, China |
| Received:October 24, 2019 |
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| KeyWord:antibiotic resistance gene;family farm;livestock waste;farmland soil |
| Author Name | Affiliation | E-mail | | GU Yan-ru | Northeast Agricultural University, Harbin 150036, China
Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | HAN Bing-jun | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | HUANG Ji-yuan | Northeast Agricultural University, Harbin 150036, China
Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | YANG Feng-xia | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | yangfengxiacomeon@163.com | | ZHANG Ke-qiang | Northeast Agricultural University, Harbin 150036, China
Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | keqiangzhang68@163.com |
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| Abstract: |
| In order to understand the occurrence characteristics and potential environmental risks of the antibiotic resistance genes(ARGs) in livestock waste from family farms, 22 typical family farms were selected to investigate the pollution characteristics of ARGs in different livestock wastes and explore the effects on surrounding farmland soils in Jizhou area of Tianjin using real-time fluorescence quantitative PCR. The results showed that the sulfonamide-, tetracyclic-, quinolone-, and macrolide-resistance genes were highly prevalent in livestock waste from these family farms. Among them, the pollution level of tetracycline resistance genes tetO, tetQ, tetW and macrolide resistance gene ermB was most severe in these family farms. In addition, β-lactam resistance gene(bla gene)that is related to human health, like blaOXA-1、blaTEM-1, and blaampC have also been detected in livestock waste. The following results were found:(1)Compared with pig waste and cattle waste, the pollution level of ARGs was more serious in chicken waste;(2)The relative abundance of most ARGs was significantly higher in sow waste than in piglet pig waste and fattening pig waste(P<0.05);(3)Application of livestock waste could significantly increase ARGs abundance in soil environment(approximately 8~18 times)(P<0.05). The above results indicate that ARGs pollution was prevalent and severe in livestock waste from family farms, which would increase the environmental risk via manure application. Finally, it may harm human health by further polluting crops; thus, ARGs contamination in family livestock farms requires more attention. |
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