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| Isolation and identification of multidrug-resistant bacteria in feces from large-scale livestock and poultry farms and their antibiotic-resistance characteristics |
| Received:July 05, 2019 |
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| KeyWord:livestock feces;multidrug-resistant bacteria;16S rDNA;antibiotic susceptibility testing;integron |
| Author Name | Affiliation | E-mail | | TANG Wei-xin | College of Forestry, Northeast Forestry University, Harbin 150040, China
Beijing Agricultural Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China | | | SUN Xing-bin | College of Forestry, Northeast Forestry University, Harbin 150040, China | sunxingbin1025@163.com | | GAO Hao-ze | Beijing Agricultural Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China | | | CHENG Shou-tao | Beijing Agricultural Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China | | | QIU Tian-lei | Beijing Agricultural Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China | qqray01@126.com | | WANG Xu-ming | Beijing Agricultural Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China | |
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| Abstract: |
| To investigate the characteristics of multidrug-resistant bacteria in livestock manure, the bacterial counts were determined in poultry manure, cow manure, swine manure, and organic fertilizer. First, the multiantibiotic-resistant bacteria were isolated and purified. Then, 16S rDNA sequence-based identification was performed, and their antibiotic susceptibilities were tested by disk diffusion susceptibility method. The absolute and relative numbers of bacteria resistant to tetracycline, enrofloxacin, sulfamethoxazole, and tylosin in manure were ranked, from highest to lowest, according to their percentage:poultry manure, swine manure and cow manure. The relative number of multidrug-resistant bacteria was lower in organic fertilizer than in manure. By the identification and taxonomic analysis of drug-resistant strains, it was found that most multidrug-resistant bacteria in livestock manure belonged to Proteobacteria, Firmicutes, and Actinobacteria; the dominant genus of multidrug-resistant bacteria were Escherichia, Corynebacterium, and Kurthia. The dominant genus of multidrug-resistant bacteria in organic fertilizer were Staphylococcus and Glutamicibacter. Antibiotic susceptibility testing of the strains showed that the multidrug-resistant bacteria in all the types of animal manure had high resistance rates to erythromycin and tetracycline, with more than 80%. Most of strains showed sensitivity to amikacin; the amikacin-resistant rate in all strains was less than 30%. The results of amplification of class Ⅰ and class Ⅱ integron gene cassettes showed that the size of PCR products ranged from 0.8 kb to 1.8 kb, and adA2, dfrA17, dfrA1, and sat2 were the majority class I integron gene cassettes in farm manure. |
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