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| Bacterial community diversity of litters at different depths in microbial fermentation bed |
| Received:April 03, 2019 |
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| KeyWord:microbial fermentation bed;bacterial diversity;high-throughput sequencing;different depths |
| Author Name | Affiliation | E-mail | | CHEN Qian-qian | Agricultural Bioresources Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China | | | LIU Bo | Agricultural Bioresources Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China | fzliubo@163.com | | ZHU Yu-jing | Agricultural Bioresources Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China | | | LIU Guo-hong | Agricultural Bioresources Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China | | | CHE Jian-mei | Agricultural Bioresources Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China | | | WANG Jie-ping | Agricultural Bioresources Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China | | | ZHENG Xue-fang | Agricultural Bioresources Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China | | | ZHANG Hai-feng | Agricultural Bioresources Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China | |
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| Abstract: |
| The main objective of this study is to investigate the bacterial communities of litters at different depths in a microbial fermentation bed(MFB), and reveal the relationship between bacterial diversity and depths of the litters. In the light of the above considerations, litters from three different depths(10 cm, 30 cm, and 50 cm)in five locations of the MFB system were collected, and their bacterial communities were evaluated using high-throughput sequencing. A total of 1 045 225 sequences were obtained, and the tested litters contained 32 phyla, 303 families, 609 genera, and 1834 operational taxonomic units(OTUs)of bacteria. The shallow litters had the highest content of bacteria while the middle litters had the greatest diversity. The microbial community was obviously related to the depth of the litters. The contents of the phyla Proteobacteria(25.9%)and Actinobacteria(10.2%)in the shallow litters were higher than they were in other litters. The middle litters had high contents of Bacteroidetes(27.8%), Proteobacteria(25.1%), and Firmicutes(17.0%). Importantly, our results showed that contents of the organic matter-decomposing bacteria, including Trueperaceae(Deinococcus-Thermus), Xanthomonadaceae (Proteobacteria), and Flavobacteriaceae(Bacteroidetes)consisted of numerous species and were in high abundance in the shallow litters. However, the contents of the phyla Bacteroidetes(33.3%)and Spirochaetes(9.2%)in the deep litters were higher than those in the shallow litters were. The deep litters had the highest content of anaerobic bacteria including Spirochaetaceae(Spirochaetes)and Saprospiraceae (Bacteroidetes). The bacterial community consisted of numerous species and the most active metabolism occurred in the shallow litters, while the contents of anaerobic bacteria increased with increasing litter depth. |
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