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| Isolation, identification, and biodegradation characteristics of two phthalic acid esters-degrading strains |
| Received:March 01, 2019 |
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| KeyWord:phthalic acid esters;isolation of degrading strain;Sphingobium xenophagum;Pseudomonas anguilliseptica |
| Author Name | Affiliation | E-mail | | PAN Qi | College of Resources & Environment, Qingdao Agricultural University, Qingdao 266109, China | | | SUN Shu | College of Resources & Environment, Qingdao Agricultural University, Qingdao 266109, China | | | ZHOU Zhen-feng | College of Resources & Environment, Qingdao Agricultural University, Qingdao 266109, China
Qingdao Research Center of Rural Environmental Engineering, Qingdao 266109, China | zfzhou@qau.edu.cn |
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| Abstract: |
| In this study, we used enrichment culture to screen highly efficient bacteria for remediation of environments contaminated by phthalic acid esters(PAEs). Accordingly, we detected two PAEs-degrading strains(RXX-2 and RXX-3)in soil samples. These two strains were identified on the basis of morphological, physiological, and 16S rDNA sequence phylogenetic analyses. Furthermore, the degradation performances of the two strains were investigated under different conditions. The results showed that RXX-2 and RXX-3 were strains of Sphingobium xenophagum and Pseudomonas anguilliseptica, respectively. The optimal conditions for PAEs degradation by strain RXX-2 were pH 8, a temperature of 30℃, a shaking speed of 175 r·min-1, and a strain dosage of 1.5%, whereas the optimal conditions for PAEs degradation by strain RXX-3 were pH 7, a temperature of 30℃, a shaking speed of 175 r·min-1, and a strain of dosage 1%. Under these optimal conditions, strain RXX-2 degraded 71.43% of di-butylphthalate(DBP) and 52.85% of di-(2-ethylhexyl) phthalate (DEHP)after 5 days, whereas RXX-3 degraded 98.98% of DBP and 62.96% of DEHP after the same period of time. Our study indicates that these two strains have potential utility as agents for the bioremediation of PAEs-contaminated environments. |
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