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| Dynamic community structure of nosZ-type denitrifying bacteria during cow manure composting |
| Received:December 06, 2018 |
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| KeyWord:dynamic aerobic compost;high throughput sequencing;nosZ-type denitrifying bacteria;RDA |
| Author Name | Affiliation | E-mail | | XU Ben-shu | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | | | MEN Meng-qi | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | | | WU Xiao-tong | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | | | JIANG Xin | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | | | SHENG Si-yuan | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | | | HAN Yue | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | | | ZHU Hai-feng | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | | | MENG Qing-xin | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | | | DENG Li-ting | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | | | XU Xiu-Hong | College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China | xuxiuhong@neau.edu.cn |
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| Abstract: |
| This study examined the dynamic nature and diversity of the community structure of the nosZ-type denitrifying bacteria with varying periods and layers during cow manure and rice straw composting. This was done via high throughput sequencing. The relationship between the denitrifying bacteria and physicochemical parameters was investigated using redundancy analysis (RDA)and Spearman correlation analysis. The results indicated that the community structure of the nosZ-type denitrifying bacteria varied significantly through the composting stages. Further, both the diversity and richness of the denitrifying bacteria displayed a declining tendency at the beginning and an increasing trend in late phase of composting. The denitrifying bacterial community structure and diversity index were greatly affected by composting depth in the late thermophilic stage. In addition, physicochemical parameters significantly affected the community structure. Significantly positive correlations were observed between Chelatococcu and temperature (P<0.01), and Polymorphum and nitrate nitrogen (P<0.01). Significantly negative correlations were seen between Mesorhizobium and moisture content and C/N (P<0.01). The diversity of the denitrifying bacterial communities was significantly negatively correlated with temperature, pH, and ammonium nitrogen (P<0.01)and significantly positively correlated with nitrate nitrogen (P<0.01). The richness of the denitrifying bacteria was significantly negatively correlated with temperature (P<0.01). In conclusion, our results indicate that time and depth are both factors influencing the nosZ-type denitrifying bacterial community structure, and the community structure is significantly affected by physicochemical parameters. This study provides an insight into the theory of the role of the nitrogen cycle in composting and thus promotes the improvement of composting technology. |
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