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Effects of long-term fertilization on soil soluble organic nitrogen and free amino acid profile variations in paddy fields
Received:October 10, 2018  
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KeyWord:long-term fertilization;paddy soil;soluble organic nitrogen;free amino acids;profile difference
Author NameAffiliationE-mail
LEI Xiu-mei College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China  
WANG Fei College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Soil and Fertilizer Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China 
 
ZHOU Bi-qing College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China
University Key Lab of Soil Ecosystem Health and Regulation, Fujian Agriculture and Forestry University, Fuzhou 350002, China 
 
YANG Wen-hao College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China
University Key Lab of Soil Ecosystem Health and Regulation, Fujian Agriculture and Forestry University, Fuzhou 350002, China 
 
NIE San-an College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China 
 
XING Shi-he College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China
University Key Lab of Soil Ecosystem Health and Regulation, Fujian Agriculture and Forestry University, Fuzhou 350002, China 
fafuxsh@126.com 
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Abstract:
      In order to investigate the effects of long-term fertilization on the profile differentiation of soluble organic nitrogen(SON)and free amino acid(FAA)in paddy soils, the leaching characteristics of SON and FAA in long-term fertilized paddy soils were determined.The SON contents, FAA amounts, and FAA types, in paddy field soil sampled at 0~20 cm, 20~40 cm, and 40~60 cm depth, with a 33-year history of different fertilization, were analyzed. The fertilization regimes included control(CK), chemical fertilizer(NPK), cow manure combined with chemical fertilizer(NPKM), and rice straw combined with chemical fertilizer(NPKS). The results showed that the profile differentiation of SON contents varied greatly in the sampled soil layers. The SON contents in the 0~20 cm layer showed the following order after different treatments:NPKS > NPKM > NPK=CK. The soil SON in the 0~20 cm layer, under NPKM and NPKS treatments, included 20 types of FAA, and showed increases in three types of basic amino acids(ornithine, lysine, and arginine), compared with CK and NPK treatments. The soil SON in the 20~40 cm layer, under NPK, NPKM, and NPKS treatments, contained 10 types of FAA, and increased levels of three neutral amino acids(proline, cystine, and phenylalanine), compared with CK treatment. The soil SON in the 40~60 cm layer, under NPK, NPKM, and NPKS treatments, contained seven types of FAA, and increased levels of two types of neutral amino acids(isoleucine and cystine), compared with CK treatment. The FAA compositions in the paddy field under different treatments were dominated by neutral amino acids. Overall, profile differentiations of SON and FAA in the paddy fields were closely related to fertilization and SON contents, and both the amounts and types of FAA in the 0~20 cm layer of the paddy soil could be increased by long-term fertilizations with NPKS and NPKM.