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| Molecular responses of Rhus chinensis to lead stress revealed by RNA-Seq |
| Received:December 11, 2018 |
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| KeyWord:Rhus chinensis;lead stress;ribosome;RNA-Seq |
| Author Name | Affiliation | E-mail | | XIA Li-dan | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Collaborative Innovation Center for Soil and Water Conservation Across the Taiwan Straits, Fuzhou 350002, China
National Positioning Observation and Research Station of Red Soil Hill Ecosystem in Changting, Fujian, Fuzhou 350002, China | | | ZHANG Hong | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Collaborative Innovation Center for Soil and Water Conservation Across the Taiwan Straits, Fuzhou 350002, China
National Positioning Observation and Research Station of Red Soil Hill Ecosystem in Changting, Fujian, Fuzhou 350002, China | | | HU Hua-ying | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Collaborative Innovation Center for Soil and Water Conservation Across the Taiwan Straits, Fuzhou 350002, China
National Positioning Observation and Research Station of Red Soil Hill Ecosystem in Changting, Fujian, Fuzhou 350002, China | | | CAO Sheng | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Collaborative Innovation Center for Soil and Water Conservation Across the Taiwan Straits, Fuzhou 350002, China
National Positioning Observation and Research Station of Red Soil Hill Ecosystem in Changting, Fujian, Fuzhou 350002, China | | | ZHOU Chui-fan | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Collaborative Innovation Center for Soil and Water Conservation Across the Taiwan Straits, Fuzhou 350002, China
1. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China | zhouchuifan@163.com |
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| Abstract: |
| Rhus chinensis is one of the pioneer plants for ecological restoration of heavy-metal-polluted areas. Since this species shows good tolerance to heavy metals such as lead and chromium, exploring its molecular response mechanism to heavy metal stress can provide a reference for the ecological restoration of heavy-metal pollution. In this study, we exposed R. chinensis to three concentrations of lead, corresponding to no, mild, and severe lead stress, respectively(0, 250, 1000 mg·kg-1), and performed transcriptome sequencing of the roots on the Illumina HiSeqTM 2000 system. Significant enrichment analysis was performed for Gene Ontology(GO)and Pathway functions, and the differentially expressed genes in response to the different lead concentrations were analyzed. Four types of hydrolases and two types of phosphatase-related genes were found to be differentially expressed in the mild lead-stress condition, which indicated that R. chinensis was mainly regulated by intracellular hydrolase and phosphatase-related genes. Under severe lead stress, six types of oxidoreductase-related genes were significantly enriched and played a major regulatory role. The cells were damaged under lead stress, and 24 and 16 cell-related genes were significantly enriched under mild and severe lead stress, respectively. However, R. chinensis could cope with this adversity by regulating cellular movements such as the movement of organelles and cytoplasm in cells. The ribosomal metabolic pathway was found to be the main metabolic pathway for salt tolerance and adaptation to lead stress, and ribosome-associated genes appeared to be the major regulatory genes induced in response to lead stress in R. chinensis. |
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