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Release law of sugarcane slag carbon source and orthogonal testing of sulfate-reducing bacteria
Received:August 02, 2018  
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KeyWord:bagasse;sulfate-reducing bacteria;reducing sugar;carbon source
Author NameAffiliation
DI Jun-zhen College of Civil Engineering, Liaoning Technical University, Fuxin 123000, China 
LI Tuo-da College of Civil Engineering, Liaoning Technical University, Fuxin 123000, China 
ZHAO Wei College of Civil Engineering, Liaoning Technical University, Fuxin 123000, China 
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Abstract:
      Aiming at the problems of high cost and easy release of secondary pollution during the use of traditional organic carbon sources, single factor experiments and orthogonal experiments were performed to study the release law of the carbon source and the optimal condition of sulfate-reducing bacteria (SRB) using bagasse. The single factor experiments were carried out at different pH values, with different bagasse particle sizes, and with different bagasse dosages. The orthogonal experiments were carried out with different SRB dosages, different bagasse particle sizes, and different bagasse dosages. The results of the single factor experiments showed that the hydrolysis rates of cellulose and glucose in bagasse were slower when the wastewater pH value was 7 and the particle size and dosage of bagasse were 60 mesh and 3.5 g·100 mL-1, respectively, which would be beneficial to the continuous accumulation of reducing sugar. The results of the orthogonal experiments showed that the removal effect of SO42- was the best and the hydrolysis rate of reducing sugar was the lowest when the SRB dosage was 71.2 mg·100 mL-1 and the particle size and dosage of bagasse were 100 mesh and 4.5 g·100 mL-1, respectively, which was determined as the best combination of orthogonal experimental conditions. Under the best combination conditions, the removal rate of SO42- and release amount of reducing sugar in the solution were still 75.23% and 64.03 mg·g-1, respectively, and the oxidation-reduction potential (ORP) was -224 mV, after the bagasse was continuously supplied as a carbon source for 96 hours.