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| Biological response of soil nirS-and nosZ-type denitrifying bacteria to dairy slurry irrigation in community structure and diversity |
| Received:July 11, 2018 |
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| KeyWord:nirS;nosZ;denitrifying bacteria;community structure;dairy slurry irrigation |
| Author Name | Affiliation | E-mail | | GAO Wen-xuan | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China | | | YAN Jian-hua | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | DU Hui-ying | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | ZHANG Ke-qiang | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | keqiangzhang68@163.com |
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| Abstract: |
| In order to provide scientific basis for dairy biogas slurry fertilizer, pilot fields in Xushui (Hebei Province, China) were treated by conventional chemical fertilizer and dairy biogas slurry with diverse concentration and frequency, for 2 years. Soil chemical properties in different soil layers were measured and the bacteria community structures of nirS-and nosZ-denitrifying bacteria were analyzed through Terminal Restriction Fragment Length Polymorphism (T-RFLP). The results indicated that the community structure of nirS-and nosZ-denitrifying bacteria were sensitive to the frequency and concentration of the applied dairy biogas slurry, except that of nirS-denitrifying bacteria in the soil of 0~20 cm. The community structures of both nirS-and nosZ-denitrifying bacteria showed significant differences in vertical distribution. Based on the T-RFLP data, three diversity indexes, namely, the Shannon-Wiener H', the Simpson D,and the Pielou E were calculated. The data indicated that different biogas slurry treatments impacted little on the diversity of nirS-denitrifiers, but had effect on the diversity of nosZ-denitrifiers. With the same N-input amount (300 kg·hm-2), high concentration of dairy slurry treatment (T5) resulted in significant higher diversity index for nosZ-denitrifiers than conventional fertilizer (CF) did. The experimental results also suggested that most of the community diversity indexes of nirS-and nosZ-denitrifying bacteria were higher in the soil of 20~40 cm than those in the soil of 0~20 cm. In this study, the community of nirS-denitrifying bacteria mainly consisted of organisms close to Azoarcus, Cupriavidus, Rubrivivax, Pseudomonas and Rhodanobacter in Betaproteobacteria, while the community of nosZ-denitrifying bacteria mainly consisted of organisms close to Rhizobium, Burkholderiales, Alcaligenes and Pseudomonas in Alphaproteobacteria, and a small part belonged to unclassified organism. |
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