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| Effect of benzo[a]pyrene on the characteristics of Mucor mucedo EPS |
| Received:July 03, 2018 |
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| KeyWord:extracellular polymer;benzo[a]pyrene;M. mucedo;physicochemical property;variation characteristic |
| Author Name | Affiliation | E-mail | | TANG Rui | School of Environment and Safety Engineering, Shenyang University of Chemical Technology, Shenyang 110142, China | | | SHAO Hong | School of Environment and Safety Engineering, Shenyang University of Chemical Technology, Shenyang 110142, China | | | JIA Chun-yun | Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China | jiachunyun@126.com | | ZHANG Zuo-jin | University of Science and Technology Liaoning, Anshan 114051, China | | | CHEN Xiang | Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
University of Chinese Academy of Sciences, Beijing 100049, China | |
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| Abstract: |
| To elucidate the role of Mucor mucedo EPS in the remediation of polycyclic aromatic hydrocarbon (PAH) pollution, the extracted concentration of EPS (TOC) and the content of proteins (PN), polysaccharides (PS), humic acid (HC), and DNA in EPS were determined after the exposure of M. mucedo to different concentrations of benzo[a]pyrene (BaP). Three-dimensional fluorescence spectroscopy (3DEEM) and flourier transform infrared spectroscopy (FTIR) were used to verify the changes in the concentration of proteins when different concentrations of BaP were used, and scanning electron microscopy (SEM) was employed to study the changes in the structure of EPS. The results showed that the changes in the concentration of TOC, PN, PS, and HC showed the same trend as the exposed BaP concentrations increased, and their content increased initially, and then decreased. When the concentration of BaP was 40 mg·L-1, the concentration of TOC, PN, PS, and HC produced by unit biomass reached the maximum values of 1 407.2 mg·g-1, 700 g·g-1, 564 g·g-1, and 174 g·g-1, respectively. According to the results of 3D-EEM, when the concentration of BaP was 40 mg·L-1, the intensity of fluorescence protein (peak A and peak B) reached the maximum value of 68.91 a.u and 245.6 a.u. The analysis of FTIR showed that compared with that of the blank control, the cytoplasmic protein amideⅠand amide Ⅱ bands of the EPS shifted to the long wave direction under the induction of BaP. The results of SEM indicated that the increase in BaP concentration gradually hardened the EPS surface, the EPS was wool-like, and the voids reached the maximum number when the concentration of BaP was 40 mg·L-1. These findings show that BaP can break the original structure of M. mucedo EPS, low concentrations of BaP can promote the production of fungus EPS, and PN and PS might play a major role during the metabolic degradation of BaP by M. mucedo. |
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